Synthetic genome recoding: new genetic codes for new features
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DOI: 10.1007/s00294-017-0754-z
Abstract: Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus… read more here.
Keywords: new genetic; genome recoding; genetic codes; synthetic genome ... See more keywords
ReScribe: An Unrestrained Tool Combining Multiplex Recombineering and Minimal-PAM ScCas9 for Genome Recoding Pseudomonas putida
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DOI: 10.1021/acssynbio.1c00297
Abstract: Genome recoding enables incorporating new functions into the DNA of microorganisms. By reassigning codons to noncanonical amino acids, the generation of new-to-nature proteins offers countless opportunities for bioproduction and biocontainment in industrial chassis. A key… read more here.
Keywords: genome recoding; pseudomonas putida; tool; minimal pam ... See more keywords
Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA
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DOI: 10.1038/s41467-020-20373-z
Abstract: While genome recoding using quadruplet codons to incorporate non-proteinogenic amino acids is attractive for biotechnology and bioengineering purposes, the mechanism through which such codons are translated is poorly understood. Here we investigate translation of quadruplet… read more here.
Keywords: quadruplet codons; genome recoding; mechanism; frameshifting trna ... See more keywords