Visualizing Single-Nucleotide Variations in a Nuclear Genome Using Colocalization of Dual-Engineered CRISPR Probes.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01208
Abstract: Direct visualization of single-nucleotide variation (SNV) in single cells is of great importance for understanding the spatial organization of genomes and their relationship with cell phenotypes. Herein, we developed a new strategy for visualizing SNVs… read more here.
Keywords: nuclear genome; colocalization; using colocalization; single nucleotide ... See more keywords
Shuffling the yeast genome using CRISPR/Cas9-generated DSBs that target the transposable Ty1 elements
Sign Up to like & getrecommendations! Published in 2023 at "PLOS Genetics"
DOI: 10.1371/journal.pgen.1010590
Abstract: Although homologous recombination between transposable elements can drive genomic evolution in yeast by facilitating chromosomal rearrangements, the details of the underlying mechanisms are not fully clarified. In the genome of the yeast Saccharomyces cerevisiae, the… read more here.
Keywords: recombination; yeast genome; cas9; shuffling yeast ... See more keywords