Molecular Characterization of a Vector-Based Candidate Antigen Using the 3'-RACE and Genome Walking Methods and In Silico Analysis of the Correspondent Protein for Vaccine Design and Development.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1884-4_29
Abstract: The main objectives of developing vaccines to prevent malaria transmission are malaria control and preventing the reemergence of the disease in endemic regions. Molecular and in silico characterization of a candidate molecule is the first… read more here.
Keywords: characterization; genome walking; vaccine design; race genome ... See more keywords
Wristwatch PCR: A Versatile and Efficient Genome Walking Strategy
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.792848
Abstract: Genome walking is a method used to retrieve unknown flanking DNA. Here, we reported wristwatch (WW) PCR, an efficient genome walking technique mediated by WW primers (WWPs). WWPs feature 5′- and 3′-overlap and a heterologous… read more here.
Keywords: pcr versatile; wristwatch pcr; genome walking; efficient genome ... See more keywords
Bridging PCR: An Efficient and Reliable Scheme Implemented for Genome-Walking
Sign Up to like & getrecommendations! Published in 2023 at "Current Issues in Molecular Biology"
DOI: 10.3390/cimb45010033
Abstract: The efficacy of the available genome-walking methods is restricted by low specificity, high background, or composite operations. We herein conceived bridging PCR, an efficient genome-walking approach. Three primers with random sequences, inner walker primer (IWP),… read more here.
Keywords: pcr efficient; genome walking; bridging pcr; non target ... See more keywords