From functional genomics of vero cells to CRISPR‐based genomic deletion for improved viral production rates
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28190
Abstract: Despite their wide use in the vaccine manufacturing field for over 40 years, one of the main limitations to recent efforts to develop Vero cells as high‐throughput vaccine manufacturing platforms is the lack of understanding… read more here.
Keywords: vero; vero cells; genomic deletion; gene ... See more keywords
Netherton syndrome caused by compound heterozygous mutation, c.80A>G mutation in SPINK5 and large‐sized genomic deletion mutation, and successful treatment of intravenous immunoglobulin
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Genetics & Genomic Medicine"
DOI: 10.1002/mgg3.1600
Abstract: Netherton syndrome (NS) is an autosomal recessive disorder due to mutations in the SPINK5 gene. Here, we report the first case of NS caused by a large genomic deletion. read more here.
Keywords: netherton syndrome; genomic deletion; syndrome caused; mutation ... See more keywords
Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion
Sign Up to like & getrecommendations! Published in 2017 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1005341
Abstract: CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of single guide RNAs (sgRNAs). This approach has been widely adopted for diverse applications, from disease modelling of individual loci, to parallelized loss-of-function… read more here.
Keywords: guide rnas; genomic deletion; scalable design; deletion ... See more keywords
Whole transcriptomic and proteomic analyses of an isogenic M. tuberculosis clinical strain with a naturally occurring 15 Kb genomic deletion
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0179996
Abstract: Tuberculosis remains one of the most difficult to control infectious diseases in the world. Many different factors contribute to the complexity of this disease. These include the ability of the host to control the infection… read more here.
Keywords: genomic deletion; natural mutant; tuberculosis; naturally occurring ... See more keywords
Variable Features of Juvenile Polyposis Syndrome With Gastric Involvement Among Patients With a Large Genomic Deletion of BMPR1A
Sign Up to like & getrecommendations! Published in 2019 at "Clinical and Translational Gastroenterology"
DOI: 10.14309/ctg.0000000000000054
Abstract: OBJECTIVES: Loss-of-function mutations of BMPR1A cause juvenile polyposis syndrome (JPS), but large genomic deletions in BMPR1A are rare, reported in few families only, and data regarding the associated phenotype are limited. METHODS: We investigated clinical… read more here.
Keywords: large genomic; genomic deletion; polyposis syndrome; deletion ... See more keywords