Whole‐genomic DNA amplifications from individually isolated sweet sorghum microspores
Sign Up to like & getrecommendations! Published in 2022 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11501
Abstract: Abstract Premise Sorghum is a multi‐use crop, the efficient breeding of which requires the development of new genetic tools. One such tool could be the genetic assessment of free microspores, which are released just after… read more here.
Keywords: whole genomic; dna; genomic dna; sorghum ... See more keywords
Biocatalytic synthesis of 2′‐deoxynucleotide 5′‐triphosphates from bacterial genomic DNA: Proof of principle
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28374
Abstract: 2′‐deoxynucleoside 5′‐triphosphates (dNTPs) are the building blocks of DNA and are key reagents which are incorporated by polymerase enzymes during nucleic acid amplification techniques, such as polymerase chain reaction (PCR). These techniques are of high… read more here.
Keywords: proof principle; dna; genomic dna; bacterial genomic ... See more keywords
Detection of Modified Bases in Bacteriophage Genomic DNA.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_5
Abstract: Collectively, the dsDNA tailed bacteriophages (Caudovirales) contain the largest chemical diversity of naturally occurring deoxynucleotides in DNA observed to date. The continuing discovery of new modifications in phages suggest many more are waiting to be… read more here.
Keywords: bases bacteriophage; bacteriophage genomic; modified bases; dna ... See more keywords
dsDNA from extracellular vesicles (EVs) in adult AML
Sign Up to like & getrecommendations! Published in 2020 at "Annals of Hematology"
DOI: 10.1007/s00277-020-04109-z
Abstract: Dear Editor, We read with great interest the article byKontopoulou et al. [1] describing the study of the dsDNA carried by extracellular vesicles (EVs) in pediatric acute myeloid leukemia (AML) patients. The authors described a… read more here.
Keywords: adult; aml patients; dna; genomic dna ... See more keywords
Metallic surface dynamics of genomic DNA and its nitrogenous bases: SERS assessment and theoretical considerations
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Molecular Modeling"
DOI: 10.1007/s00894-019-4039-y
Abstract: The dynamics of genomic DNA and its nucleobases at a silver surface were explored using surface-enhanced Raman spectroscopy (SERS) and ab initio Verlet-type dynamics, respectively. The bands observed had full widths at half-maximum (FWHMs) in… read more here.
Keywords: genomic dna; dynamics genomic; dna nitrogenous; surface dynamics ... See more keywords
Testing the impact of Whole Genome Amplification on genome comparison using the polyploid flagellated Giardia duodenalis as a model.
Sign Up to like & getrecommendations! Published in 2019 at "Experimental parasitology"
DOI: 10.1016/j.exppara.2019.107776
Abstract: The availability of high quality genomic DNA in sufficient amounts to perform Next Generation Sequencing (NGS) experiments is challenging for pathogens that cannot be cultivated in vitro, as is the case for many parasites. Therefore,… read more here.
Keywords: whole genome; genomic dna; duodenalis model; genome amplification ... See more keywords
Development of direct assays for Toxoplasma gondii and its use in genomic DNA sample
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2017.07.050
Abstract: Graphical abstract Figure. No Caption available. HighlightsA new biosensor to toxoplasmosis was developed based on modified electrode.The biosensor was able of detect until 100 ng mL−1 of the T. gondii genomic DNA.The genosensor showed high… read more here.
Keywords: gondii genomic; dna; biosensor; genomic dna ... See more keywords
"MINES" method for genomic DNA extraction from deep biosphere biofilms.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2019.105730
Abstract: Successful and efficient extraction of high quality, high molecular weight genomic DNA from the environmental samples is an essential primary step to understand genetic, metabolic and evolutionary characteristics of microbial communities. Deep mine biofilm samples… read more here.
Keywords: extraction; dna; method genomic; genomic dna ... See more keywords
Impact of the central atom on human genomic DNA and human serum albumin binding properties in analogous Zn(II) and Cd(II) complexes with mefenamic acid
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Molecular Structure"
DOI: 10.1016/j.molstruc.2019.03.078
Abstract: Abstract Two novel complexes of Zn(II) and Cd(II) with Hmef (mefenamic acid) and bapen (N,N′-bis(3-aminopropyl)ethylenediamine) ligand were synthesized and characterized by infrared spectroscopy, elemental, thermal and single-crystal X-ray structure analysis. Both complexes form molecular structure… read more here.
Keywords: dna; mefenamic acid; central atom; human serum ... See more keywords
Electro-DBS: A Simple Method to Rapidly Extract Genomic DNA from Dried Blood Spots.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02021
Abstract: Dried blood spots (DBSs) have been used for more than 50 years and are used as a method of sample collection for pharmacological testing, genetic analyses, monitoring of viral infections, and more. Protocols for nucleic… read more here.
Keywords: extract genomic; dna; genomic dna; dried blood ... See more keywords
LC-MS/MS for Assessing the Incorporation and Repair of N2-Alkyl-2'-deoxyguanosine in Genomic DNA.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical research in toxicology"
DOI: 10.1021/acs.chemrestox.2c00101
Abstract: Understanding the occurrence, repair, and biological consequences of DNA damage is important in environmental toxicology and risk assessment. The most common way to assess DNA damage elicited by exogenous sources in a laboratory setting is… read more here.
Keywords: toxicology; dna; genomic dna; assessing incorporation ... See more keywords