An in vitro system for measuring genotoxicity mediated by human CYP3A4 in Saccharomyces cerevisiae
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DOI: 10.1002/em.22093
Abstract: P450 activity is required to metabolically activate many chemical carcinogens, rendering them highly genotoxic. CYP3A4 is the most abundantly expressed P450 enzyme in the liver, accounting for most drug metabolism and constituting 50% of all… read more here.
Keywords: saccharomyces cerevisiae; genotoxicity; human cyp3a4; yeast ... See more keywords
The in vitro genotoxicity potency of mixtures of pyrrolizidine alkaloids can be explained by dose addition of the individual mixture components
Sign Up to like & getrecommendations! Published in 2022 at "Environmental and Molecular Mutagenesis"
DOI: 10.1002/em.22512
Abstract: Plant‐based 1,2‐unsaturated Pyrrolizidine Alkaloids (PAs) are responsible for liver genotoxicity/carcinogenicity following metabolic activation, making them a relevant concern for safety assessment. Due to 21st century toxicology approaches, risk of PAs can be better discerned though… read more here.
Keywords: potency; vitro genotoxicity; genotoxic potency; genotoxicity potency ... See more keywords
Establishing a quantitative framework for regulatory interpretation of genetic toxicity dose–response data: Margin of exposure case study of 48 compounds with both in vivo mutagenicity and carcinogenicity dose–response data
Sign Up to like & getrecommendations! Published in 2022 at "Environmental and Molecular Mutagenesis"
DOI: 10.1002/em.22517
Abstract: Quantitative relationships between carcinogenic potency and mutagenic potency have been previously examined using a benchmark dose (BMD)‐based approach. We extended those analyses by using human exposure data for 48 compounds to calculate carcinogenicity‐derived and genotoxicity‐derived… read more here.
Keywords: carcinogenicity; response data; dose response; genotoxicity ... See more keywords
Quantitative in vitro to in vivo extrapolation of genotoxicity data provides protective estimates of in vivo dose
Sign Up to like & getrecommendations! Published in 2022 at "Environmental and Molecular Mutagenesis"
DOI: 10.1002/em.22521
Abstract: Genotoxicity assessment is a critical component in the development and evaluation of chemicals. Traditional genotoxicity assays (i.e., mutagenicity, clastogenicity, and aneugenicity) have been limited to dichotomous hazard classification, while other toxicity endpoints are assessed through… read more here.
Keywords: genotoxicity data; genotoxicity; vitro vivo; vivo extrapolation ... See more keywords
Assessing the Quality and Making Appropriate Use of Historical Negative Control Data: A Report of the International Workshop on Genotoxicity Testing (IWGT).
Sign Up to like & getrecommendations! Published in 2023 at "Environmental and molecular mutagenesis"
DOI: 10.1002/em.22541
Abstract: Historical negative control data (HCD) have played an increasingly important role in interpreting the results of genotoxicity tests. In particular, Organisation for Economic Co-operation and Development (OECD) genetic toxicology Test Guidelines recommend comparing responses produced… read more here.
Keywords: genotoxicity testing; hcd; genotoxicity; negative control ... See more keywords
Interlaboratory validation of the ToxTracker assay: An in vitro reporter assay for mechanistic genotoxicity assessment
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DOI: 10.1002/em.22592
Abstract: ToxTracker is a mammalian cell reporter assay that predicts the genotoxic properties of compounds with high accuracy. By evaluating induction of various reporter genes that play a key role in relevant cellular pathways, it provides… read more here.
Keywords: interlaboratory validation; toxtracker; genotoxicity; reporter assay ... See more keywords
Assessing the genotoxicity of N‐nitrosodiethylamine with three in vivo endpoints in male Big Blue® transgenic and wild‐type C57BL/6N mice
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DOI: 10.1002/em.22615
Abstract: The detection of N‐nitrosamines in drug products has raised global regulatory interest in recent years due to the carcinogenic potential of some nitrosamines in animals and a need to identify a testing strategy has emerged.… read more here.
Keywords: mice; c57bl mice; three vivo; big blue ... See more keywords
Review and meta-analysis of gene expression biomarkers predictive of chemical-induced genotoxicity in vivo.
Sign Up to like & getrecommendations! Published in 2025 at "Environmental and molecular mutagenesis"
DOI: 10.1002/em.22646
Abstract: There is growing recognition across broad sectors of the toxicology community that gene expression biomarkers have the potential to identify genotoxic and nongenotoxic carcinogens through a weight-of-evidence approach, providing opportunities to reduce reliance on the… read more here.
Keywords: expression biomarkers; gene expression; biomarkers predictive; gene ... See more keywords
Fertilized Avian Egg Fetal Liver Assays for Assessing DNA Damaging Potential of Chemicals: A Comparative Analysis With In Vitro and In Vivo Genotoxicity Assays and Rodent Carcinogenicity
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DOI: 10.1002/em.70016
Abstract: The ability to produce direct DNA damage (genotoxicity), which underlies the carcinogenicity of various chemicals, is typically evaluated in a regulatory‐approved battery of in vitro tests with potential in vivo follow‐up. Growing concerns for animal… read more here.
Keywords: vitro; genotoxicity assays; vivo genotoxicity; avian egg ... See more keywords
The comet assay in Folsomia candida: A suitable approach to assess genotoxicity in collembolans.
Sign Up to like & getrecommendations! Published in 2017 at "Environmental toxicology and chemistry"
DOI: 10.1002/etc.3795
Abstract: The present study shows the comet assay technique being successfully applied for the first time to one of the most widely used soil organisms in standardized ecotoxicological tests, Folsomia candida, providing a step forward in… read more here.
Keywords: comet assay; methodology; folsomia candida; assay folsomia ... See more keywords
In vitro genotoxicity assessment of an oxidized dextrin‐based hydrogel for biomedical applications
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Applied Toxicology"
DOI: 10.1002/jat.3754
Abstract: Hydrogels are three‐dimensional, crosslinked networks of hydrophilic polymers swollen with a large amount of water or biological fluids, without dissolving. Dextrin, a low‐molecular‐weight carbohydrate composed by glucose residues, has been used to develop an injectable… read more here.
Keywords: based hydrogel; hydrogel biomedical; genotoxicity; dextrin based ... See more keywords