Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.202300653
Abstract: Geobacillus kaustophilus TSCCA02, a newly isolated strain from cassava (Manihot esculenta L.) rhizosphere soil in Thailand, showed maximum raw starch degrading enzyme (RSDE) activity at 252.3 ± 9.32 U/mL with cassava starch and peptone at 5.0 and 3.0 g/L,… read more here.
Keywords: amylase; geobacillus kaustophilus; kaustophilus tscca02; raw starch ... See more keywords
Structural and functional characterization of a putative carbonic anhydrase from Geobacillus kaustophilus reveals its cambialistic function.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.02.036
Abstract: Carbonic anhydrases (CA) are the most ubiquitous ancient zinc metalloenzymes known. Here we report the structural and functional analysis of a hypothetical protein GK2848 from Geobacillus kaustophilus. The analysis revealed that it belongs to the… read more here.
Keywords: geobacillus kaustophilus; functional characterization; active site; characterization putative ... See more keywords
A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus
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DOI: 10.1186/s12934-018-0969-9
Abstract: BackgroundBacterial strains of the genus Geobacillus grow at high temperatures of 50–75 °C and could thus be useful for biotechnological applications. However, genetic manipulation of these species is difficult because the current techniques for transforming Geobacillus… read more here.
Keywords: pls20cat; novel method; geobacillus kaustophilus; mobilization ... See more keywords
A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Cell Factories"
DOI: 10.1186/s12934-022-01759-8
Abstract: Background Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred… read more here.
Keywords: geobacillus kaustophilus; pls20cat orit; bacillus subtilis; conjugation ... See more keywords