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Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.02.005
Abstract: Previously, we developed a kinase cycling method using creatine kinase and pyruvate kinase (RMPK) both from rabbit muscle in the presence of an excess amount of ATP and IDP for the quantitative determination of substrate.…
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Keywords:
pyruvate kinase;
mn2;
geobacillus stearothermophilus;
reaction ... See more keywords
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Published in 2018 at "Innovative Food Science and Emerging Technologies"
DOI: 10.1016/j.ifset.2018.06.011
Abstract: Abstract Lethality rates of Bacillus subtilis, Geobacillus stearothermophilus, Alicyclobacillus acidoterrestris, and Aspergillus niger spores were studied into phosphate-buffer saline (PBS) and PBS supplemented with caramel (PBSC) after Ultra-High Pressure Homogenization (UHPH) and UV-C treatments applied…
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Keywords:
combination;
stearothermophilus alicyclobacillus;
geobacillus stearothermophilus;
niger ... See more keywords
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Published in 2019 at "International journal of food microbiology"
DOI: 10.1016/j.ijfoodmicro.2019.108334
Abstract: In this study, we show that phosphate decreases the spore heat resistance by accelerating the rate of loss of cations from spores. Heat resistance of spores of Geobacillus stearothermophilus A1, D1, P3 and ATCC 12980…
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Keywords:
resistance spores;
heat resistance;
geobacillus stearothermophilus;
phosphate ... See more keywords
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Published in 2019 at "International Journal of Heat and Mass Transfer"
DOI: 10.1016/j.ijheatmasstransfer.2019.118649
Abstract: Abstract Steam sterilization processes are used to sterilize dental handpieces before reuse. Computational Fluid Dynamics (CFD) simulations can be a useful tool to investigate the various physical phenomena that occur within steam sterilizers. In this…
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Keywords:
geobacillus stearothermophilus;
steam sterilizer;
inactivation;
dental handpieces ... See more keywords
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Published in 2021 at "BMC Biotechnology"
DOI: 10.1186/s12896-021-00678-7
Abstract: Background Proteases are important for hydrolysis of proteins to generate peptides with many bioactivities. Thus, the development of novel proteases with high activities is meaningful to discover bioactive peptides. Because natural isolation from animal, plant…
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Keywords:
alkaline serine;
geobacillus stearothermophilus;
protease;
serine protease ... See more keywords
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Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1178748
Abstract: Geobacillus stearothermophilus is a highly thermophilic, spore-forming Gram-positive bacterium that causes flat sour spoilage in low-acid canned foods. To address this problem, we isolated G. stearothermophilus-infecting phage GR1 from the soil and characterized its endolysin…
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Keywords:
endolysin;
lysgr1 ead;
geobacillus stearothermophilus;
gr1 lysgr1 ... See more keywords
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Published in 2023 at "Genes"
DOI: 10.3390/genes14020466
Abstract: The genus Geobacillus comprises thermophilic gram-positive bacteria which are widely distributed, and their ability to withstand high temperatures makes them suitable for various applications in biotechnology and industrial production. Geobacillus stearothermophilus H6 is an extremely…
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Keywords:
protease;
comparative genomic;
geobacillus stearothermophilus;
genomic analysis ... See more keywords
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Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21030735
Abstract: The conserved Histidine 301 in switch II of Geobacillus stearothermophilus IF2 G2 domain was substituted with Ser, Gln, Arg, Leu and Tyr to generate mutants displaying different phenotypes. Overexpression of IF2H301S, IF2H301L and IF2H301Y in…
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Keywords:
switch geobacillus;
disparate phenotypes;
geobacillus stearothermophilus;
initiation ... See more keywords