Single Point Mutation Abolishes Water Capture in Germacradien‐4‐ol Synthase
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DOI: 10.1002/cbic.202400290
Abstract: The high‐fidelity sesquiterpene cyclase (−)‐germacradien‐4‐ol synthase (GdolS) converts farnesyl diphosphate into the macrocyclic alcohol (−)‐germacradien‐4‐ol. Site‐directed mutagenesis was used to decipher the role of key residues in the water control mechanism. Replacement of Ala176, located… read more here.
Keywords: germacradien; germacradien synthase; water; point mutation ... See more keywords
Redesigning the Molecular Choreography to Prevent Hydroxylation in Germacradien-11-ol Synthase Catalysis
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DOI: 10.1021/acscatal.0c04647
Abstract: Natural sesquiterpene synthases have evolved to make complex terpenoids by quenching reactive carbocations either by proton transfer or by hydroxylation (water capture), depending on their active site. Germacradien-11-ol synthase (Gd11olS) from Streptomyces coelicolor catalyzes the… read more here.
Keywords: molecular choreography; germacradien synthase; germacradien; hydroxylation ... See more keywords