Generation of Pecam1 endothelial specific dual reporter mouse model.
Sign Up to like & getrecommendations! Published in 2020 at "Genesis"
DOI: 10.1002/dvg.23384
Abstract: Endothelial cells are specialized epithelium lining the interior surface of vessels and play fundamental roles in angiogenesis, vascular permeability, and immune response. To identify endothelial cells in vivo, we constructed a Pecam1nlacZ-H2B-GFP/+ knock-in mouse model… read more here.
Keywords: reporter mouse; gfp; endothelial cells; mouse model ... See more keywords
Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody
Sign Up to like & getrecommendations! Published in 2022 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202200028
Abstract: Animal‐derived anti‐IgG secondary antibodies are currently employed to stain and screen of human monoclonal antibody(mAb)‐producing cells, but using animal‐derived antibodies may raise the concerns of high cost, complicated operations and biological safety issues in biopharmaceutical… read more here.
Keywords: monoclonal antibody; producing cho; animal derived; gfp ... See more keywords
Regulation of ATF1 and ATF2 transcripts by sequences in their 3′ untranslated region in cleavage‐stage cattle embryos
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.22785
Abstract: The sequence of a 3′ untranslated region (3′UTR) of mRNA governs the timing of its polyadenylation and translation in mammalian oocytes and early embryos. The objective of this study was to assess the influence of… read more here.
Keywords: atf1 atf2; gfp; translation; atf2 transcripts ... See more keywords
Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7169-5_8
Abstract: Many genetic tools have been developed that use green fluorescent protein (GFP) and its derivatives for labeling specific cell populations in organisms and in cell culture. To extend the use of GFP beyond labeling purposes,… read more here.
Keywords: mouse brain; gfp dependent; gfp; viral delivery ... See more keywords
High-Throughput Protein-Protein Interaction Assays Using Tripartite Split-GFP Complementation.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_20
Abstract: Most cellular processes are driven by complex protein-protein interaction networks. Identifying key players and characterizing their interactions at the cellular and molecular level is of key importance to understand biochemical mechanisms that control cellular responses.… read more here.
Keywords: protein interaction; protein protein; gfp; interaction ... See more keywords
Protein engineering for covalent immobilization and enhanced stability through incorporation of multiple noncanonical amino acids
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-017-0127-y
Abstract: In this study, we demonstrate the application of multiple functional properties of proteins generated through coupling of residue-specific and site-specific incorporation method. With green fluorescent protein (GFP) as a model protein, we constructed multifunctional GFP… read more here.
Keywords: incorporation; stability; protein engineering; gfp ... See more keywords
Overexpression of Constitutively Active OsRab11 in Plants Enhances Tolerance to High Salinity Levels
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Plant Biology"
DOI: 10.1007/s12374-018-0048-0
Abstract: Rab proteins are key regulators of intracellular trafficking between specific compartments in a cell. Among them, Rab11, a widely conserved sub-group, mainly regulates plasma membrane (PM) trafficking. Previously, we reported that Oryza sativa Rab11 (OsRab11)… read more here.
Keywords: osrab11; constitutively active; tolerance high; stress ... See more keywords
GFP tagging based method to analyze the genome editing efficiency of CRISPR/Cas9-gRNAs through transient expression in N. benthamiana
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Plant Biochemistry and Biotechnology"
DOI: 10.1007/s13562-019-00540-0
Abstract: The CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR—associated proteins 9) is simple and highly efficient technology applied to functional studies of genes and genetic crop improvement. In this study, we have demonstrated the utility of… read more here.
Keywords: genome editing; editing efficiency; crispr cas9; gfp ... See more keywords
Identification of an RNA silencing suppressor encoded by citrus vein enation virus
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Plant Pathology"
DOI: 10.1007/s42161-018-0163-2
Abstract: In order to know if any protein encoded by citrus vein enation virus (CVEV) might act as an RNA silencing suppressor (RSS), five open reading frames (ORF) of CVEV, including the coat protein (CP) gene,… read more here.
Keywords: encoded citrus; rna silencing; gfp; silencing suppressor ... See more keywords
Rational engineering of a human GFP-like protein scaffold for humanized targeted nanomedicines.
Sign Up to like & getrecommendations! Published in 2021 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2021.06.001
Abstract: Green fluorescent protein (GFP) is a widely used scaffold for protein-based targeted nanomedicines because of its high biocompatibility, biological neutrality and outstanding structural stability. However, being immunogenicity a major concern in the development of drug… read more here.
Keywords: protein; protein scaffold; gfp like; like protein ... See more keywords
Melittin-Induced Permeabilization, Re-sealing, and Re-permeabilization of E. coli Membranes.
Sign Up to like & getrecommendations! Published in 2018 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.10.046
Abstract: The permeabilization of model lipid bilayers by cationic peptides has been studied extensively over decades, with the bee-sting toxin melittin perhaps serving as the canonical example. However, the relevance of these studies to the permeabilization… read more here.
Keywords: cell; induced permeabilization; gfp; melittin induced ... See more keywords