The contribution of specific subsites to catalytic activities in active site architecture of a GH11 xylanase.
Sign Up to like & getrecommendations! Published in 2020 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-020-10865-9
Abstract: BACKGROUND Xylanase with high specific activity plays a crucial role in hemicellulose biodegradation and has important industrial application. The amino acids located in the active site determine the enzyme biological characterization. In this study, structure… read more here.
Keywords: amino acids; site architecture; active site; site ... See more keywords
Increase in the thermostability of GH11 xylanase XynJ from Bacillus sp. strain 41M-1 using site saturation mutagenesis.
Sign Up to like & getrecommendations! Published in 2019 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2019.109363
Abstract: GH11 xylanase XynJ from Bacillus sp. strain 41M-1 has a β-jellyroll fold composed of eight β strands with a deep active-site cleft. We hypothesized that the thermostability of XynJ will increase if the flexibility of… read more here.
Keywords: xylanase xynj; xynj; site; gh11 xylanase ... See more keywords
Heterologous expression in Pichia pastoris and characterization of a novel GH11 xylanase from saline-alkali soil with excellent tolerance to high pH, high salt concentrations and ethanol.
Sign Up to like & getrecommendations! Published in 2017 at "Protein expression and purification"
DOI: 10.1016/j.pep.2017.06.003
Abstract: A GH11 xylanase gene (xyn11-1) cloned from saline-alkali soil was successfully expressed in Pichia pastoris GS115. The purified recombinant Xyn11-1 showed its maximal activity at pH 6.0, and retained more than 60.4% of activity at… read more here.
Keywords: xylanase; alkali soil; saline alkali; xyn11 ... See more keywords
Insights Into the Role of Exposed Surface Charged Residues in the Alkali-Tolerance of GH11 Xylanase
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2020.00872
Abstract: Thermostable and alkaline- or acid-stable xylanases are more advantageous in agricultural and industrial fields. In this study, a rational structure-based design was conducted based on a thermostable GH11 xylanase TlXynA from Thermomyces lanuginosus to improved… read more here.
Keywords: tolerance; insights role; gh11 xylanase; surface charged ... See more keywords