Optimization of the Inverted Emulsion Method for High‐Yield Production of Biomimetic Giant Unilamellar Vesicles
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201900529
Abstract: In the field of bottom‐up synthetic biology, lipid vesicles provide an important role in the construction of artificial cells. Giant unilamellar vesicles (GUVs), due to their membrane's similarity to natural biomembranes, have been widely used… read more here.
Keywords: inverted emulsion; production; method; biology ... See more keywords
Effects of Charged Lipids on Giant Unilamellar Vesicle Fusion and Inner Content Mixing via Freeze‐Thawing
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200550
Abstract: Fusion between giant unilamellar vesicles (GUVs) can incorporate and mix components of biochemical reactions. Recently, GUV fusion induced by freeze‐thawing (F/T) was employed to construct artificial cells that can easily and repeatedly fuse GUVs with… read more here.
Keywords: fusion; content mixing; effects charged; charged lipids ... See more keywords
Magainin-H2 effects on the permeabilization and mechanical properties of giant unilamellar vesicles.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of colloid and interface science"
DOI: 10.1016/j.jcis.2019.06.028
Abstract: Among the potential novel therapeutics to treat bacterial infections, antimicrobial peptides (AMPs) are a very promising substitute due to their broad-spectrum activity and rapid bactericidal action. AMPs strongly interact with the bacterial membrane, and the… read more here.
Keywords: lipid bilayers; mag; magainin effects; mechanical properties ... See more keywords
Enhancing membrane modulus of giant unilamellar lipid vesicles by lateral co-assembly of amphiphilic triblock copolymers.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of colloid and interface science"
DOI: 10.1016/j.jcis.2019.10.109
Abstract: We report a facile, but robust approach to fabricate structurally stable giant unilamellar vesicles (GUVs), on which a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayer membrane was made rigid by introducing amphiphilic block polymers. Particularly, we found that lateral… read more here.
Keywords: amphiphilic triblock; assembly amphiphilic; lateral assembly; membrane ... See more keywords
Multicomponent and Multiphase Lipid Nanotubes Formed by Gliding Microtubule-Kinesin Motility and Phase-Separated Giant Unilamellar Vesicles
Sign Up to like & getrecommendations! Published in 2019 at "Langmuir"
DOI: 10.1021/acs.langmuir.9b02637
Abstract: Cytoskeletal filaments and motor proteins are critical components in the transport and reorganization of membrane-based organelles in eukaryotic cells. Previous studies have recapitulated the microtubule-kinesin transport system in vitro to dynamically assemble large-scale nanotube networks… read more here.
Keywords: microtubule kinesin; unilamellar vesicles; phase separated; phase ... See more keywords
Wrapping Pathways of Anisotropic Dumbbell Particles by Giant Unilamellar Vesicles
Sign Up to like & getrecommendations! Published in 2023 at "Nano Letters"
DOI: 10.1021/acs.nanolett.3c00375
Abstract: Endocytosis is a key cellular process involved in the uptake of nutrients, pathogens, or the therapy of diseases. Most studies have focused on spherical objects, whereas biologically relevant shapes can be highly anisotropic. In this… read more here.
Keywords: unilamellar vesicles; giant unilamellar; anisotropic dumbbell; wrapping pathways ... See more keywords
In Situ Assembly of Transmembrane Proteins from Expressed and Synthetic Components in Giant Unilamellar Vesicles.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00013
Abstract: Reconstituting functional transmembrane (TM) proteins into model membranes is challenging due to the difficulty of expressing hydrophobic TM domains, which often require stabilizing detergents that can perturb protein structure and function. Recent model systems solve… read more here.
Keywords: transmembrane proteins; assembly transmembrane; expressed synthetic; situ assembly ... See more keywords
DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00407
Abstract: Giant Unilamellar Vesicles (GUVs) are cell-sized aqueous compartments enclosed by a phospholipid bilayer. Due to their cell-mimicking properties, GUVs have become a widespread experimental tool in synthetic biology to study membrane properties and cellular processes.… read more here.
Keywords: unilamellar vesicles; microscopy; analysis; giant unilamellar ... See more keywords
Control of Enzyme Reaction Initiation inside Giant Unilamellar Vesicles by the Cell-Penetrating Peptide-Mediated Translocation of Cargo Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00413
Abstract: Cell-penetrating peptides (CPPs) play important roles in directly delivering biomolecules, such as DNA, proteins, and peptides, into living cells. In artificial lipid membranes, such as planar lipid bilayers, the direct membrane translocation of β-galactosidase via… read more here.
Keywords: translocation; unilamellar vesicles; guvs; microscopy ... See more keywords
Extracellular Cues Govern Shape and Cytoskeletal Organization in Giant Unilamellar Lipid Vesicles
Sign Up to like & getrecommendations! Published in 2023 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00516
Abstract: Spontaneous and induced front-rear polarization and a subsequent asymmetric actin cytoskeleton is a crucial event leading to cell migration, a key process involved in a variety of physiological and pathological conditions such as tissue development,… read more here.
Keywords: lipid vesicles; unilamellar lipid; organization; cytoskeleton ... See more keywords
Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles
Sign Up to like & getrecommendations! Published in 2023 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00684
Abstract: One of the main drivers within the field of bottom-up synthetic biology is to develop artificial chemical machines, perhaps even living systems, that have programmable functionality. Numerous toolkits exist to generate giant unilamellar vesicle-based artificial… read more here.
Keywords: cell; encapsulation; encapsulation efficiency; giant unilamellar ... See more keywords