An efficient method to clone TAL effector genes from Xanthomonas oryzae using Gibson assembly
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DOI: 10.1111/mpp.12820
Abstract: Summary Transcription Activator‐Like effectors (TALes) represent the largest family of type III effectors among pathogenic bacteria and play a critical role in the process of infection. Strains of Xanthomonas oryzae pv. oryzae (Xoo) and some… read more here.
Keywords: tale genes; tale; xanthomonas oryzae; gibson assembly ... See more keywords
Gibson assembly interposition improves amplification efficiency of long DNA and multifragment overlap extension PCR.
Sign Up to like & getrecommendations! Published in 2023 at "BioTechniques"
DOI: 10.2144/btn-2023-0012
Abstract: For difficult overlap extension PCR, a Gibson assembly process was inserted between the two PCR rounds to facilitate the formation of complete gene templates at a moderate temperature. That is, after amplifying each DNA fragment,… read more here.
Keywords: overlap extension; gibson assembly; extension pcr; pcr ... See more keywords