Optimisation of droplet digital PCR for determining copy number variation of α-gliadin genes in mutant and gene-edited polyploid bread wheat
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DOI: 10.1016/j.jcs.2019.102903
Abstract: Targeted and random mutagenesis of gene families require accurate quantification. Droplet digital PCR (ddPCR) enables high-throughput screening of copy number variation (CNV). We tested the accuracy of ddPCR for CNV analysis in the large α-gliadin… read more here.
Keywords: ddpcr; gliadin genes; droplet digital; wheat ... See more keywords