Testing the CRISPR-Cas9 and glmS ribozyme systems in Leishmania tarentolae.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular and biochemical parasitology"
DOI: 10.1016/j.molbiopara.2020.111336
Abstract: Leishmania parasites include important pathogens and model organisms and are even used for the production of recombinant proteins. However, functional genomics and the characterization of essential genes are often limited in Leishmania because of low-throughput… read more here.
Keywords: knock; glms ribozyme; leishmania tarentolae; crispr cas9 ... See more keywords
The GlcN6P cofactor plays multiple catalytic roles in the glmS ribozyme.
Sign Up to like & getrecommendations! Published in 2017 at "Nature chemical biology"
DOI: 10.1038/nchembio.2300
Abstract: RNA enzymes (ribozymes) have remarkably diverse biological roles despite having limited chemical diversity. Protein enzymes enhance their reactivity through recruitment of cofactors; likewise, the naturally occurring glmS ribozyme uses the glucosamine-6-phosphate (GlcN6P) organic cofactor for… read more here.
Keywords: glms ribozyme; glcn6p cofactor; catalytic roles; plays multiple ... See more keywords
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15540-1
Abstract: Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its application to… read more here.
Keywords: sequence function; glms ribozyme; sequence; rna ... See more keywords