Multiple thermostable enzyme hydrolases on magnetic nanoparticles: An immobilized enzyme-mediated approach to saccharification through simultaneous xylanase, cellulase and amylolytic glucanotransferase action.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.09.106
Abstract: Microbe-derived enzymes such as xylanases, cellulases and amylases, are efficient at hydrolyzing plant biomass. Efforts to harness the functionalities of these enzymes towards applications in energy and fuel biosciences, and food and nutrition, continue apace… read more here.
Keywords: xylanase; magnetic nanoparticles; immobilized enzyme; cellulase ... See more keywords
Efficient production of aggregation prone 4-α-glucanotransferase by combined use of molecular chaperones and chemical chaperones in Escherichia coli.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2019.01.014
Abstract: In this study, a combined optimization strategy, based on co-expression of molecular chaperones and supplementation of osmolytes, was used to reduce the formation of inclusion bodies and enhance the expression of the soluble form of… read more here.
Keywords: aggregation prone; production; molecular chaperones; cell ... See more keywords
GtfC Enzyme of Geobacillus sp. 12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c03475
Abstract: Starch-acting α-glucanotransferase enzymes are of great interest for applications in the food industry. In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or… read more here.
Keywords: represents novel; gtfc enzyme; geobacillus 12amor1; gtfc ... See more keywords
Acceptor dependent catalytic properties of GH57 4-α-glucanotransferase from Pyrococcus sp. ST04
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.1016675
Abstract: The 4-α-glucanotransferase (4-α-GTase or amylomaltase) is an essential enzyme in maltodextrin metabolism. Generally, most bacterial 4-α-GTase is classified into glycoside hydrolase (GH) family 77. However, hyperthermophiles have unique 4-α-GTases belonging to GH family 57. These… read more here.
Keywords: pyrococcus st04; catalytic properties; glucanotransferase; family ... See more keywords
Biochemical Characterization of Alkaliphilic Cyclodextran Glucanotransferase from an Alkaliphilic Bacterium, Paenibacillus daejeonensis.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of microbiology and biotechnology"
DOI: 10.4014/jmb.1810.10007
Abstract: Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system… read more here.
Keywords: paenibacillus daejeonensis; daejeonensis; biochemical characterization; citase ... See more keywords