Hitting the sweet spot with capillary electrophoresis: advances in N-glycomics and glycoproteomics.
Sign Up to like & getrecommendations! Published in 2021 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2021.07.013
Abstract: N-glycosylation is of paramount importance for understanding the mechanisms of various human diseases and ensuring the safety and efficacy of biotherapeutics. Traditional glycan analysis techniques include LC-based separations and MALDI-TOF-MS identification. However, the current state-of-the-art… read more here.
Keywords: capillary electrophoresis; hitting sweet; sweet spot; glycomics glycoproteomics ... See more keywords
A rapid and facile preparation of APTS-labeled N-glycans by combination of ion pair-assisted extraction and HILIC-SPE for routine glycan analysis.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113875
Abstract: Glycoanalytical technology is required for a wide variety of scientific research, including basic glycobiological pharmaceutical, and biomarker research. Although several innovative analytical techniques have been developed for these purposes, quantitative glycan analysis based on electrophoretic… read more here.
Keywords: labeled glycans; analysis; apts labeled; hilic spe ... See more keywords
In a pursuit of optimal glycan fluorescent label for negative MS mode for high-throughput N-glycan analysis
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2022.999770
Abstract: Over the past few decades, essential role of glycosylation in protein functioning has become widely recognized, rapidly advancing glycan analysis techniques. Because free glycan’s lack chromophore or fluorophore properties, and do not ionize well, they… read more here.
Keywords: negative mode; glycan analysis; fluorescent; high throughput ... See more keywords