(Glycan Binding) Activity-Based Protein Profiling in Cells Enabled by Mass Spectrometry-Based Proteomics
Sign Up to like & getrecommendations! Published in 2023 at "Israel journal of chemistry"
DOI: 10.1002/ijch.202200097
Abstract: The presence of glycan modifications at the cell surface and other locales positions them as key regulators of cell recognition and function. However, due to the complexity of glycosylation, the annotation of which proteins bear… read more here.
Keywords: glycan binding; mass spectrometry; activity based; based protein ... See more keywords
Glycan-binding profile of DC-like cells
Sign Up to like & getrecommendations! Published in 2019 at "Glycoconjugate Journal"
DOI: 10.1007/s10719-019-09897-9
Abstract: Modification of vaccine carriers by decoration with glycans can enhance binding to and even targeting of dendritic cells (DCs), thus augmenting vaccine efficacy. To find a specific glycan-“vector” it is necessary to know glycan-binding profile… read more here.
Keywords: glycan binding; dcs; cd14 low; binding profile ... See more keywords
The Glycan Microarray Story from Construction to Applications.
Sign Up to like & getrecommendations! Published in 2017 at "Accounts of chemical research"
DOI: 10.1021/acs.accounts.7b00043
Abstract: Not only are glycan-mediated binding processes in cells and organisms essential for a wide range of physiological processes, but they are also implicated in various pathological processes. As a result, elucidation of glycan-associated biomolecular interactions… read more here.
Keywords: glycan binding; construction; glycan associated; glycan microarray ... See more keywords
Mass Spectrometry-based Shotgun Glycomics for Discovery of Natural Ligands of Glycan-binding Proteins.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c02931
Abstract: Glycans attached to lipids and membrane-bound and secreted proteins and peptides mediate many important physiological and pathophysiological processes through interactions with glycan-binding proteins (GBPs). However, uncovering functional glycan ligands is challenging due to the large… read more here.
Keywords: glycan binding; mass spectrometry; binding proteins; glycan ligands ... See more keywords
Engineered Glycan-Binding Proteins for Recognition of the Thomsen-Friedenreich Antigen and Structurally Related Disaccharides.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00683
Abstract: Glycan-binding proteins (GBPs) are widely used reagents for basic research and clinical applications. These reagents allow for the identification and manipulation of glycan determinants without specialized equipment or time-consuming experimental methods. Existing GBPs, mainly antibodies… read more here.
Keywords: glycan binding; friedenreich antigen; binding proteins; thomsen friedenreich ... See more keywords
Cell-based glycan arrays for probing glycan–glycan binding protein interactions
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DOI: 10.1038/s41467-018-03245-5
Abstract: Glycan microarrays provide a high-throughput means of profiling the interactions of glycan-binding proteins with their ligands. However, the construction of current glycan microarray platforms is time consuming and expensive. Here, we report a fast and… read more here.
Keywords: cell based; glycan binding; based glycan; glycan arrays ... See more keywords
Biomimetic nanochannels for the discrimination of sialylated glycans via a tug-of-war between glycan binding and polymer shrinkage†‡
Sign Up to like & getrecommendations! Published in 2019 at "Chemical Science"
DOI: 10.1039/c9sc05319k
Abstract: Sialylated glycans that are attached to cell surface mediate diverse cellular processes such as immune responses, pathogen binding, and cancer progression. Precise determination of sialylated glycans, particularly their linkage isomers that can trigger distinct biological… read more here.
Keywords: glycan binding; tug war; biomimetic nanochannels; shrinkage ... See more keywords
Systematic synthesis of bisected N-glycans and unique recognitions by glycan-binding proteins
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Science"
DOI: 10.1039/d1sc05435j
Abstract: Bisected N-glycans represent a unique class of protein N-glycans that play critical roles in many biological processes. Herein, we describe the systematic synthesis of these structures. A bisected N-glycan hexasaccharide was chemically assembled with two… read more here.
Keywords: glycan binding; bisected glycans; binding proteins; systematic synthesis ... See more keywords
Glycans and Glycan-Binding Proteins in Atherosclerosis.
Sign Up to like & getrecommendations! Published in 2019 at "Thrombosis and haemostasis"
DOI: 10.1055/s-0039-1692720
Abstract: Complex glycans are readily accessible on the endothelium and on cell and plasma components. They interact with glycan-binding proteins which translate their structure into function. Advanced analytical tools are available to investigate their structure and… read more here.
Keywords: proteins atherosclerosis; glycan binding; glycans glycan; binding proteins ... See more keywords
The Glycan-Binding Trait of the Sarbecovirus Spike N-Terminal Domain Reveals an Evolutionary Footprint
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Virology"
DOI: 10.1128/jvi.00958-22
Abstract: Spike N-terminal domains (S-NTD) of sarbecoviruses are highly diverse; however, their function remains largely understudied compared with the receptor-binding domains (RBD). Here, we show that sarbecovirus S-NTD can be phylogenetically clustered into five clades and… read more here.
Keywords: glycan binding; cell; sarbecovirus; spike terminal ... See more keywords
Recent Progress in Clinical Development of Therapeutic Antibodies Targeting Glycan-Binding Proteins.
Sign Up to like & getrecommendations! Published in 2018 at "Current drug targets"
DOI: 10.2174/1389450119666180308144313
Abstract: BACKGROUND Glycan-binding proteins are widely distributed in human and play an essential role in biological processes. Their involvements in inflammatory and immune responses make it increasingly likely that the glycan-binding proteins may represent valuable therapeutic… read more here.
Keywords: glycan binding; therapeutic antibodies; targeting glycan; binding proteins ... See more keywords