Fractionated plasma N‐glycan profiling of novel cohort of ATP6AP1‐CDG subjects identifies phenotypic association
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Inherited Metabolic Disease"
DOI: 10.1002/jimd.12589
Abstract: ATP6AP1‐CDG is an X‐linked disorder typically characterized by hepatopathy, immunodeficiency, and an abnormal type II transferrin glycosylation pattern. Here, we present 11 new patients and clinical updates with biochemical characterization on one previously reported patient.… read more here.
Keywords: plasma glycan; glycan profiling; atp6ap1 cdg; fractionated plasma ... See more keywords
High-throughput and high-sensitivity N-Glycan profiling: a platform for biopharmaceutical development and disease biomarker discovery.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114205
Abstract: Protein glycosylation contributes to critical biological function of glycoproteins. Glycan analysis is essential for the production of biopharmaceuticals as well as for the identification of biomarkers of disease. However, glycans are highly heterogeneous, which has… read more here.
Keywords: glycan profiling; biomarker discovery; high sensitivity; biopharmaceutical development ... See more keywords
At-line N-linked glycan profiling for monoclonal antibodies with advanced sample preparation and high-performance liquid chromatography.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2020.04.009
Abstract: N-linked glycosylation is a post-translational modification that occurs on many proteins during biosynthesis. The profile of different glycans on the protein is a critical quality attribute of some recombinant biopharmaceutical proteins including monoclonal antibodies (mAbs).… read more here.
Keywords: sample preparation; glycan profiling; preparation; liquid chromatography ... See more keywords
Differential N-glycan patterns identified in lung adenocarcinoma by N-glycan profiling of formalin-fixed paraffin-embedded (FFPE) tissue sections.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2017.11.010
Abstract: N-glycan profiling is a powerful approach for analyzing the functional relationship between N-glycosylation and cancer. Current methods rely on either serum or fresh tissue samples; however, N-glycan patterns may differ between serum and tissue, as… read more here.
Keywords: lung adenocarcinoma; glycan profiling; glycan patterns; tissue ... See more keywords
Validation of Rapi-Fluor method for glycan profiling and application to commercial antibody drugs.
Sign Up to like & getrecommendations! Published in 2019 at "Talanta"
DOI: 10.1016/j.talanta.2019.01.093
Abstract: N-glycans influence the activity of antibody drugs such as antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Thus, glycan profiling is considered a critical quality attribute (CQA) and requires routine and comprehensive monitoring. In this… read more here.
Keywords: glycan profiling; antibody drugs; method; fluor method ... See more keywords
Differentiation of Sialyl Linkages Using a Combination of Alkyl Esterification and Phenylhydrazine Derivatization: Application for N-Glycan Profiling in the Sera of Patients with Lung Cancer.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00105
Abstract: Alterations in oligosaccharides and types of sialic acid (SA) attachments have been associated with different pathological states. Matrix-assisted laser desorption mass spectrometry (MS) is commonly used for glycosylation studies. However, native sialylated glycans are suppressed… read more here.
Keywords: lung cancer; alkyl esterification; glycan profiling; profiling sera ... See more keywords
Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01030
Abstract: Hepatitis B virus (HBV) is a double-stranded DNA virus composed of three types of viral particles. The virions are called Dane particles and the others are noninfectious subviral particles (SVPs). In blood, SVPs are detected… read more here.
Keywords: glycan profiling; dane particles; dane; hbv ... See more keywords
A Rapid Array-Based Approach to N-Glycan Profiling of Cultured Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00303
Abstract: Typically, N-glycosylation studies done on cultured cells require up to millions of cells followed by lengthy preparation to release, isolate, and profile N-glycans. To overcome these limitations, we report a rapid array-based workflow for profiling… read more here.
Keywords: profiling; glycan profiling; cultured cells; approach glycan ... See more keywords
Serum N-Glycan Profiling of Patients with Narcolepsy Type 1 Using LC-MS/MS
Sign Up to like & getrecommendations! Published in 2024 at "ACS Omega"
DOI: 10.1021/acsomega.4c01593
Abstract: The neurological condition known as narcolepsy type 1 (NT1) is an uncommon condition marked by extreme daytime sleepiness, cataplexy, sleep paralysis, hallucinations, disrupted nocturnal sleep, and low or undetectable levels of orexin in the CSF… read more here.
Keywords: glycan biomarkers; glycan profiling; profiling patients; narcolepsy type ... See more keywords
A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies
Sign Up to like & getrecommendations! Published in 2024 at "mAbs"
DOI: 10.1080/19420862.2024.2304268
Abstract: ABSTRACT Glycosylation plays a crucial role in determining the quality and efficacy of therapeutic antibodies. This necessitates a thorough analysis and monitoring process to ensure consistent product quality during manufacturing. In this study, we introduce… read more here.
Keywords: microarray; lectin microarray; glycan profiling; tailored lectin ... See more keywords