In vitro glycation of a tissue‐engineered wound healing model to mimic diabetic ulcers
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28359
Abstract: Diabetic foot ulcers are a major complication of diabetes that occurs following minor trauma. Diabetes‐induced hyperglycemia is a leading factor inducing ulcer formation and manifests notably through the accumulation of advanced glycation end‐products (AGEs) such… read more here.
Keywords: wound healing; glycation; model; healing model ... See more keywords
Kinetic modeling as a tool to understand the influence of cell culture process parameters on the glycation of monoclonal antibody biotherapeutics
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2865
Abstract: Glycation, the nonenzymatic reaction between the reducing sugar glucose and the primary amine residues on amino acid side chains, commonly occurs in the cell culture supernatant during production of therapeutic monoclonal antibodies (mAbs). While glycation… read more here.
Keywords: temperature; cell culture; process; glycation ... See more keywords
Antibody glycation during a CHO fed-batch process following a constrained second order reaction.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology progress"
DOI: 10.1002/btpr.3261
Abstract: Glycation on lysine side chains of recombinant monoclonal antibodies (mAb) is a well-known phenomenon in manufacturing processes of biopharmaceuticals that potentially alter the efficacy of the therapeutic protein. In the present study, we report kinetic… read more here.
Keywords: cho fed; fed batch; order; reaction ... See more keywords
Nonenzymatic glycosylation of isolated human immunoglobulin‐G by D‐ribose
Sign Up to like & getrecommendations! Published in 2022 at "Cell Biochemistry and Function"
DOI: 10.1002/cbf.3722
Abstract: Glycation is vital in terms of its damaging effect on macromolecules resulting in the formation of end products, which are highly reactive and cross‐linked irreversible structures, known as advanced glycation end products (AGEs). The continuous… read more here.
Keywords: glycosylation isolated; glycation; isolated human; human immunoglobulin ... See more keywords
Metformin encapsulated gold nanoparticles (MTF‐GNPs): A promising antiglycation agent
Sign Up to like & getrecommendations! Published in 2022 at "Cell Biochemistry and Function"
DOI: 10.1002/cbf.3738
Abstract: The generation of advanced glycation end products (AGEs) through nonenzymatic protein glycation contributes to the pathogenesis of long‐lived diabetic problems. Metformin (MTF) is the very first drug having antihyperglycemic effects on type II diabetes mellitus… read more here.
Keywords: mtf gnps; gold nanoparticles; antiglycation; glycation ... See more keywords
Assessing glycation-mediated changes in human cortical bone with Raman spectroscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biophotonics"
DOI: 10.1002/jbio.201700352
Abstract: Establishing a non-destructive method for spatially assessing advanced glycation end-products (AGEs) is a potentially useful step toward investigating the mechanistic role of AGEs in bone quality. To test the hypothesis that the shape of the… read more here.
Keywords: raman spectroscopy; cortical bone; spectroscopy; glycation ... See more keywords
HPLC–free method of synthesis of isotopically labeled deoxyfructosylated peptides
Sign Up to like & getrecommendations! Published in 2022 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-022-04022-6
Abstract: The biomarker strategy, based on multiple specific glycation sites in plasma proteins, could essentially increase the efficiency of glycemic control and disease prediction. Besides glycated albumin being a potential biomarker of early states of diabetes… read more here.
Keywords: labeled deoxyfructosylated; synthesis isotopically; glycation; method ... See more keywords
Silver Nanoparticles Synthesized Using Eysenhardtia polystachya and Assessment of the Inhibition of Glycation in Multiple Stages In Vitro and in the Zebrafish Model
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cluster Science"
DOI: 10.1007/s10876-018-1448-5
Abstract: The aim was to investigate the inhibitory activities on AGE formation by testing silver nanoparticles fabricated using a methanol extract of Eysenhardtia polystachya (EP–AgNPs) and characterized using various physicochemical techniques. The in vitro glucose-albumin assay… read more here.
Keywords: silver nanoparticles; nanoparticles synthesized; eysenhardtia polystachya; glycation ... See more keywords
Acceleration of protein glycation by oxidative stress and comparative role of antioxidant and protein glycation inhibitor
Sign Up to like & getrecommendations! Published in 2019 at "Molecular and Cellular Biochemistry"
DOI: 10.1007/s11010-019-03550-7
Abstract: Hyperglycemia in diabetes causes protein glycation that leads to oxidative stress, release of cytokines, and establishment of secondary complications such as neuropathy, retinopathy, and nephropathy. Several other metabolic disorders, stress, and inflammation generate free radicals… read more here.
Keywords: oxidative stress; protein glycation; glycation inhibitor; glycation ... See more keywords
Destructive effect of non-enzymatic glycation on catalase and remediation via curcumin.
Sign Up to like & getrecommendations! Published in 2017 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2017.06.018
Abstract: Non-enzymatic glycation of proteins is a post-translational modification that is produced by a covalent binding between reducing sugars and amino groups of lysine and arginine residues. In this paper the effect of pathological conditions, derived… read more here.
Keywords: enzymatic glycation; effect; non enzymatic; glycation ... See more keywords
Intensification of serum albumin amyloidogenesis by a glycation-peroxidation loop (GPL).
Sign Up to like & getrecommendations! Published in 2019 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2019.05.008
Abstract: The interaction of reducing sugars with proteins leads to the formation of advanced glycation end products (AGE) and reactive oxidative species (ROS). ROS peroxidise free or membrane included unsaturated fatty acids, leading to generate reactive… read more here.
Keywords: peroxidation loop; peroxidation; glycation; loop gpl ... See more keywords