Metabolic Glycan Labeling in Primary Neurons Enabled by Unnatural Sugars with No S-Glyco-Modification.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.3c00152
Abstract: It is of great interest to probe glycosylation in primary neuron cultures. However, per-O-acetylated clickable unnatural sugars, which have been routinely utilized in metabolic glycan labeling (MGL) for analyzing glycans, showed cytotoxicity to cultured primary… read more here.
Keywords: metabolic glycan; primary neurons; unnatural sugars; glyco modification ... See more keywords
Protein S-Glyco-Modification Through an Elimination-Addition Mechanism.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.0c02110
Abstract: Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades but it is only until recently that we discovered the existence of an… read more here.
Keywords: protein; mechanism; addition; glyco modification ... See more keywords
Enzymatic Glyco-Modification of Synthetic Membrane Systems
Sign Up to like & getrecommendations! Published in 2023 at "Biomolecules"
DOI: 10.3390/biom13020335
Abstract: The present report assesses the capability of a soluble glycosyltransferase to modify glycolipids organized in two synthetic membrane systems that are attractive models to mimic cell membranes: giant unilamellar vesicles (GUVs) and supported lipid bilayers… read more here.
Keywords: synthetic membrane; modification synthetic; membrane systems; glyco modification ... See more keywords