Initiating base excision repair in chromatin.
Sign Up to like & getrecommendations! Published in 2018 at "DNA repair"
DOI: 10.1016/j.dnarep.2018.08.011
Abstract: The base excision repair (BER) pathway removes modified nucleobases that can be deleterious to an organism. BER is initiated by a glycosylase, which finds and removes these modified nucleobases. Most of the characterization of glycosylase… read more here.
Keywords: base excision; excision repair; glycosylase; modified nucleobases ... See more keywords
An effective human uracil-DNA glycosylase inhibitor targets the open pre-catalytic active site conformation.
Sign Up to like & getrecommendations! Published in 2021 at "Progress in biophysics and molecular biology"
DOI: 10.1016/j.pbiomolbio.2021.02.004
Abstract: Human uracil DNA-glycosylase (UDG) is the prototypic and first identified DNA glycosylase with a vital role in removing deaminated cytosine and incorporated uracil and 5-fluorouracil (5-FU) from DNA. UDG depletion sensitizes cells to high APOBEC3B… read more here.
Keywords: uracil dna; inhibitor; dna; dna glycosylase ... See more keywords
Characterization of a Novel Thermostable DNA Lyase Used To Prepare DNA for Next-Generation Sequencing
Sign Up to like & getrecommendations! Published in 2023 at "Chemical Research in Toxicology"
DOI: 10.1021/acs.chemrestox.2c00172
Abstract: Recently, we constructed a hybrid thymine DNA glycosylase (hyTDG) by linking a 29-amino acid sequence from the human thymine DNA glycosylase with the catalytic domain of DNA mismatch glycosylase (MIG) from M. thermoautotrophicum, increasing the… read more here.
Keywords: dna; glycosylase; activity; next generation ... See more keywords
A silver nanorod based SERS assay for the homogeneous detection of uracil-DNA glycosylase activity
Sign Up to like & getrecommendations! Published in 2017 at "Analytical Methods"
DOI: 10.1039/c6ay02869a
Abstract: As an important base-excision repair enzyme, much evidence has shown that uracil-deoxyribonucleic acid glycosylase (UDG) plays a crucial role in maintaining the genomic integrity. This work developed a surface-enhanced Raman scattering (SERS) method for the… read more here.
Keywords: detection; homogeneous detection; surface; glycosylase ... See more keywords
Base excision repair of the N-(2-deoxy-d-erythro-pentofuranosyl)-urea lesion by the hNEIL1 glycosylase
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad164
Abstract: Abstract The N-(2-deoxy-d-erythro-pentofuranosyl)-urea DNA lesion forms following hydrolytic fragmentation of cis-5R,6S- and trans-5R,6R-dihydroxy-5,6-dihydrothymidine (thymine glycol, Tg) or from oxidation of 7,8-dihydro-8-oxo-deoxyguanosine (8-oxodG) and subsequent hydrolysis. It interconverts between α and β deoxyribose anomers. Synthetic oligodeoxynucleotides… read more here.
Keywords: urea lesion; glycosylase; lesion; hneil1 ... See more keywords
Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gky162
Abstract: Abstract Base excision repair (BER), which is initiated by DNA N-glycosylase proteins, is the frontline for repairing potentially mutagenic DNA base damage. The NTHL1 glycosylase, which excises DNA base damage caused by reactive oxygen species,… read more here.
Keywords: overexpression; base excision; glycosylase; cancer ... See more keywords
Programmable deaminase-free base editors for G-to-Y conversion by engineered glycosylase
Sign Up to like & getrecommendations! Published in 2023 at "National Science Review"
DOI: 10.1093/nsr/nwad143
Abstract: Current DNA base editors contain nuclease and DNA deaminase that enables deamination of cytosine (C) or adenine (A), but no method for guanine (G) or thymine (T) editing is available now. Here we developed a… read more here.
Keywords: programmable deaminase; deaminase free; glycosylase; base editors ... See more keywords
Interaction of Thymine DNA Glycosylase with Oxidised 5-Methyl-cytosines in Their Amino- and Imino-Forms
Sign Up to like & getrecommendations! Published in 2021 at "Molecules"
DOI: 10.3390/molecules26195728
Abstract: Thymine DNA Glycosylase (TDG) is an enzyme of the base excision repair mechanism and removes damaged or mispaired bases from DNA via hydrolysis of the glycosidic bond. Specificity is of high importance for such a… read more here.
Keywords: oxidised methyl; amino; dna; imino ... See more keywords