Enhanced glycosylation of an S‐layer protein enables a psychrophilic methanogenic archaeon to adapt to elevated temperatures in abundant substrates
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13650
Abstract: Adaptation to higher temperatures would increase the environmental competitiveness of psychrophiles, organisms that thrive in low‐temperature environments. Methanolobus psychrophilus, a cold wetland methanogen, ‘evolved’ as a mesophile, growing optimally at 30 °C after subculturings, and… read more here.
Keywords: glycosylation layer; glycosylation; layer protein; archaeon ... See more keywords
N‐glycosylation and expression in human tissues of the orphan GPR61 receptor
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12339
Abstract: A number of members of the G protein‐coupled receptor class of cell surface receptors are ‘orphans’ with no known endogenous ligand. One of these orphan receptors is GPR61; there are little data about its expression… read more here.
Keywords: gpr61; expression human; glycosylation; expression ... See more keywords
DPAGT1‐Mediated Protein N‐Glycosylation Is Indispensable for Oocyte and Follicle Development in Mice
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.202000531
Abstract: Abstract Post‐translational modification of proteins by N‐linked glycosylation is crucial for many life processes. However, the exact contribution of N‐glycosylation to mammalian female reproduction remains largely undefined. Here, DPAGT1, the enzyme that catalyzes the first… read more here.
Keywords: indispensable oocyte; protein glycosylation; dpagt1; glycosylation ... See more keywords
FKRP directed fibronectin glycosylation: A novel mechanism giving insights into muscular dystrophies?
Sign Up to like & getrecommendations! Published in 2022 at "BioEssays"
DOI: 10.1002/bies.202100270
Abstract: The recently uncovered role of Fukutin‐related protein (FKRP) in fibronectin glycosylation has challenged our understanding of the basis of disease pathogenesis in the muscular dystrophies. FKRP is a Golgi‐resident glycosyltransferase implicated in a broad spectrum… read more here.
Keywords: muscular dystrophies; fibronectin glycosylation; directed fibronectin; dystrophies fkrp ... See more keywords
An integrated modular framework for modeling the effect of ammonium on the sialylation process of monoclonal antibodies produced by CHO cells
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology Journal"
DOI: 10.1002/biot.202100019
Abstract: Monoclonal antibodies (mABs) have emerged as one of the most important therapeutic recombinant proteins in the pharmaceutical industry. Their immunogenicity and therapeutic efficacy are influenced by post‐translational modifications, specifically the glycosylation process. Bioprocess conditions can… read more here.
Keywords: integrated modular; modular framework; monoclonal antibodies; process ... See more keywords
N‐glycan engineering of a plant‐produced anti‐CD20‐hIL‐2 immunocytokine significantly enhances its effector functions
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26503
Abstract: Anti‐CD20 recombinant antibodies are among the most promising therapeutics for the treatment of B‐cell malignancies such as non‐Hodgkin lymphomas. We recently demonstrated that an immunocytokine (2B8‐Fc‐hIL2), obtained by fusing an anti‐CD20 scFv‐Fc antibody derived from… read more here.
Keywords: plant; effector functions; glycosylation; anti cd20 ... See more keywords
Probabilistic model by Bayesian network for the prediction of antibody glycosylation in perfusion and fed‐batch cell cultures
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27769
Abstract: Glycosylation is a critical quality attribute of therapeutic monoclonal antibodies (mAbs). The glycan pattern can have a large impact on the immunological functions, serum half‐life and stability. The medium components and cultivation parameters are known… read more here.
Keywords: network; bayesian network; glycosylation; perfusion fed ... See more keywords
Differential N- and O-glycosylation signatures of HIV-1 Gag virus-like particles and coproduced extracellular vesicles.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28051
Abstract: HIV-1 virus-like particles (VLPs) are nanostructures derived from the self-assembly and cell budding of Gag polyprotein. Mimicking the native structure of the virus and being non-infectious, they represent promising candidates for the development of new… read more here.
Keywords: coproduced extracellular; virus like; glycosylation; extracellular vesicles ... See more keywords
Strategies to control therapeutic antibody glycosylation during bioprocessing: Synthesis and separation
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28066
Abstract: Glycosylation can be a critical quality attribute in biologic manufacturing. In particular, it has implications on the half‐life, immunogenicity, and pharmacokinetics of therapeutic monoclonal antibodies (mAbs), and must be closely monitored throughout drug development and… read more here.
Keywords: therapeutic antibody; antibody glycosylation; glycosylation; downstream ... See more keywords
Augmenting glycosylation‐directed folding pathways enhances the fidelity of HIV Env immunogen production in plants
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28169
Abstract: Heterologous glycoprotein production relies on host glycosylation‐dependent folding. When the biosynthetic machinery differs from the usual expression host, there is scope to remodel the assembly pathway to enhance glycoprotein production. Here we explore the integration… read more here.
Keywords: hiv; glycosylation directed; directed folding; production ... See more keywords
Cell culture process metabolomics together with multivariate data analysis tools opens new routes for bioprocess development and glycosylation prediction
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Progress"
DOI: 10.1002/btpr.3012
Abstract: Multivariate latent variable methods have become a popular and versatile toolset to analyze bioprocess data in industry and academia. This work spans such applications from the evaluation of the role of the standard process variables… read more here.
Keywords: cell culture; process; glycosylation; data analysis ... See more keywords