Synthesis of lipid-linked oligosaccharides by a compartmentalized multi-enzyme cascade for the in vitro N-glycosylation of peptides.
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DOI: 10.1016/j.jbiotec.2020.07.003
Abstract: A wide range of glycoproteins can be recombinantly expressed in aglycosylated forms in bacterial and cell-free production systems. To investigate the effect of glycosylation of these proteins on receptor binding, stability, efficacy as drugs, pharmacodynamics… read more here.
Keywords: glycosylation peptides; vitro glycosylation; multi enzyme; cascade ... See more keywords