Enzymatic hydrolysis of cellulosic materials using synthetic mixtures of purified cellulases bioengineered at N-glycosylation sites
Sign Up to like & getrecommendations! Published in 2018 at "3 Biotech"
DOI: 10.1007/s13205-018-1419-4
Abstract: Mutant forms of recombinant endoglucanase II (EG II, N194A), cellobiohydrolase I (CBH I, N45A) and cellobiohydrolase II (CBH II, N219A) from Penicillium verruculosum with enhanced cellulase activities, achieved by engineering of enzyme N-glycosylation sites in… read more here.
Keywords: hydrolysis cellulosic; hydrolysis; cbh cbh; glycosylation sites ... See more keywords
N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings.
Sign Up to like & getrecommendations! Published in 2021 at "Veterinary microbiology"
DOI: 10.1016/j.vetmic.2021.109215
Abstract: Duck Tembusu virus (DTMUV) is an emerging mosquito-borne flavivirus that has caused acute egg-drop syndrome in egg-laying ducks. DTMUV nonstructural protein 1 (NS1) contains three potential predicted N-linked glycosylation sites at residues 130, 175 and… read more here.
Keywords: n130 n175; n175 n207; linked glycosylation; ns1 ... See more keywords
HIV-1 subtype CRF01_AE and B differ in utilization of low levels of CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
Sign Up to like & getrecommendations! Published in 2017 at "Virology"
DOI: 10.1016/j.virol.2017.09.026
Abstract: HIV subtypes not only predominate in different geographical regions but also differ in key phenotypic characteristics. To determine if genotypic and/or phenotypic differences in the Envelope (Env) glycoprotein can explain subtype related differences, we cloned… read more here.
Keywords: potential glycosylation; glycosylation sites; levels ccr5; subtype ... See more keywords
Precise O-Glycosylation Site Localization of CD24Fc by LC-MS Workflows.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01137
Abstract: CD24Fc is a homodimeric recombinant Fc-fusion protein comprised of human CD24 connected to immunoglobulin G1 (IgG1) Fc fragment. CD24 is heavily glycosylated, and its biological function is considered mainly mediated by its glycosylation. Identification of… read more here.
Keywords: glycosylation site; precise glycosylation; glycosylation; glycosylation sites ... See more keywords
Recombinant Human Lysyl Oxidase-like 2 Secreted from Human Embryonic Kidney Cells Displays Complex and Acidic Glycans at All Three N-Linked Glycosylation Sites.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.7b00849
Abstract: Human lysyl oxidase-like 2 (hLOXL2), a glycoprotein implicated in tumor progression and organ fibrosis, is a molecular target for anticancer and antifibrosis treatment. This glycoprotein contains three predicted N-linked glycosylation sites; one is near the… read more here.
Keywords: protein; oxidase like; glycosylation; lysyl oxidase ... See more keywords
Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases
Sign Up to like & getrecommendations! Published in 2018 at "Nature Chemical Biology"
DOI: 10.1038/s41589-018-0051-2
Abstract: AbstractGlycosylation is an abundant post-translational modification that is important in disease and biotechnology. Current methods to understand and engineer glycosylation cannot sufficiently explore the vast experimental landscapes required to accurately predict and design glycosylation sites… read more here.
Keywords: rapid synthesis; sites rapid; glycosylation; design glycosylation ... See more keywords
Machupo Virus with Mutations in the Transmembrane Domain and Glycosylation Sites of the Glycoprotein Is Attenuated and Immunogenic in Animal Models of Bolivian Hemorrhagic Fever
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Virology"
DOI: 10.1128/jvi.00209-22
Abstract: For arenaviruses, the only vaccine available is the live attenuated Candid#1 vaccine, a JUNV vaccine approved in Argentina. We and others have found that the glycans on GPC and the F427 residue in the GPC… read more here.
Keywords: macv; gpc tmd; glycosylation sites; vaccine ... See more keywords
Prediction of N-linked glycosylation sites using position relative features and statistical moments
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0181966
Abstract: Glycosylation is one of the most complex post translation modification in eukaryotic cells. Almost 50% of the human proteome is glycosylated as glycosylation plays a vital role in various biological functions such as antigen’s recognition,… read more here.
Keywords: sites using; linked glycosylation; glycosylation; using position ... See more keywords
Nglyc: A random forest method for prediction of N-Glycosylation sites in eukaryotic protein sequence.
Sign Up to like & getrecommendations! Published in 2019 at "Protein and peptide letters"
DOI: 10.2174/0929866526666191002111404
Abstract: N-Glycosylation is one of the most important post-translational mechanisms in eukaryotes. It plays a vital role in various biological processes such as protein folding, stability, immunogenicity of different proteins, cell signaling and protein targeting. N-glycosylation… read more here.
Keywords: protein; glycosylation; prediction glycosylation; sequence ... See more keywords
Structural and Quantitative Characterization of Mucin-Type O-Glycans and the Identification of O-Glycosylation Sites in Bovine Submaxillary Mucin
Sign Up to like & getrecommendations! Published in 2020 at "Biomolecules"
DOI: 10.3390/biom10040636
Abstract: Bovine submaxillary mucin (BSM) is a gel-forming glycoprotein polymer, and Ser/Thr-linked glycans (O-glycans) are important in regulating BSM’s viscoelasticity and polymerization. However, details of O-glycosylation have not been reported. This study investigates the structural and… read more here.
Keywords: bovine submaxillary; submaxillary mucin; glycosylation; galnac ... See more keywords
Different N-Glycosylation Sites Reduce the Activity of Recombinant DSPAα2
Sign Up to like & getrecommendations! Published in 2022 at "Current Issues in Molecular Biology"
DOI: 10.3390/cimb44090270
Abstract: Bat plasminogen activators α2 (DSPAα2) has extremely high medicinal value as a powerful natural thrombolytic protein. However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause… read more here.
Keywords: reduce activity; different glycosylation; glycosylation; glycosylation sites ... See more keywords