Biochemical characterization of an organic solvent‐tolerant glycosyltransferase from Bacillus licheniformis PI15 with potential application for raspberry ketone glycoside production
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1841
Abstract: Raspberry ketone is a primary aroma component of the red raspberry. The glycosylation of this compound is a potential approach used to improve its pharmaceutical properties. In this work, raspberry ketone glycosides are produced in… read more here.
Keywords: solvent tolerant; raspberry ketone; raspberry; glycosyltransferase ... See more keywords
FAM3B/PANDER-Like Carbohydrate-Binding Domain in a Glycosyltransferase, POMGNT1.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0430-4_52
Abstract: Protein O-mannose β1,2-N-acetylglucosaminyltransferase 1 (POMGNT1) is one of the gene products responsible for α-dystroglycanopathy, which is a type of congenital muscular dystrophy caused by O-mannosyl glycan defects. The originally identified function of POMGNT1 was as… read more here.
Keywords: pomgnt1; carbohydrate binding; domain; glycosyltransferase ... See more keywords
In Vitro Characterization of a Multidomain Glycosyltransferase Using Fluorescently Tagged Synthetic Acceptors.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9154-9_19
Abstract: In vitro assays using fluorescently tagged sugar residues can facilitate the characterization of glycosyltransferase function. Here we describe the use of in vitro assays to characterize the three glycosyltransferase modules of the protein designated WbbB… read more here.
Keywords: vitro characterization; using fluorescently; glycosyltransferase; synthetic acceptors ... See more keywords
Genome-wide identification and expression analysis of glycosyltransferase gene family 1 in Quercus robur L.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of applied genetics"
DOI: 10.1007/s13353-021-00650-3
Abstract: Glycosyltransferase gene family 1, also known as uridine diphosphate glycosyltransferase (UGT), is the largest glycosyltransferase family in plants, playing a vital role in their growth and development. In this study, 244 UGT genes with conserved… read more here.
Keywords: ugt genes; analysis; family; expression ... See more keywords
Molecular cloning and biochemical characterization of a new flavonoid glycosyltransferase from the aquatic plant lotus.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.01.099
Abstract: Lotus (Nelumbo nucifera Gaertn.), one of the earliest plants in angiosperms, is a perennial aquatic herb widely distributed throughout Eastern Asia. Quercetin and its glycosides are the most abundant phenolic compounds in lotus with multiple… read more here.
Keywords: aquatic plants; lotus; glycosyltransferase aquatic; glycosyltransferase ... See more keywords
High level production of flavonoid rhamnosides by metagenome-derived Glycosyltransferase C in Escherichia coli utilizing dextrins of starch as a single carbon source.
Sign Up to like & getrecommendations! Published in 2019 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2019.07.002
Abstract: Flavonoids exert a wide variety of biological functions that are highly attractive for the pharmaceutical and healthcare industries. However, their application is often limited by low water solubility and poor bioavailability, which can generally be… read more here.
Keywords: high level; production; escherichia coli; metagenome derived ... See more keywords
Enhancement of Rebaudioside M Production by Structure-Guided Engineering of Glycosyltransferase UGT76G1.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c01209
Abstract: Owing to zero-calorie and advanced organoleptic properties similar to sucrose, the plant-derived rebaudioside M (Reb M) has been considered as a next generation sweetener. However, a low content of Reb M in Stevia rebaudiana Bertoni… read more here.
Keywords: ugt76g1 t284s; production; glycosyltransferase; structure guided ... See more keywords
UGT74AN1, a Permissive Glycosyltransferase from Asclepias curassavica for the Regiospecific Steroid 3-O-Glycosylation.
Sign Up to like & getrecommendations! Published in 2018 at "Organic letters"
DOI: 10.1021/acs.orglett.7b03619
Abstract: A permissive steroid glycosyltransferase (UGT74AN1) from Asclepias curassavica exhibited robust capabilities for the regiospecific C3 glycosylation of cardiotonic steroids and C21 steroid precursors, and unprecedented promiscuity toward 53 structurally diverse natural and unnatural compounds to… read more here.
Keywords: glycosylation; asclepias curassavica; glycosyltransferase; steroid glycosylation ... See more keywords
Highly Promiscuous Flavonoid 3- O-Glycosyltransferase from Scutellaria baicalensis.
Sign Up to like & getrecommendations! Published in 2019 at "Organic letters"
DOI: 10.1021/acs.orglett.9b00524
Abstract: A highly regio-specific and donor-promiscuous 3- O-glycosyltransferase, Sb3GT1 (UGT78B4), was discovered from Scutellaria baicalensis. Sb3GT1 could accept five sugar donors (UDP-Glc/-Gal/-GlcNAc/-Xyl/-Ara) to catalyze 3- O-glycosylation of 17 flavonols, and the conversion rates could be >98%.… read more here.
Keywords: promiscuous flavonoid; scutellaria baicalensis; highly promiscuous; flavonoid glycosyltransferase ... See more keywords
Fabricating Bimetal Organic Material Capsules with a Commodious Microenvironment and Synergistic Effect for Glycosyltransferase.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c04644
Abstract: Metal-organic frameworks (MOFs) are rarely applied as solid supports in the enzymatic synthesis of oligosaccharides and polysaccharides, as glycosyltransferases are readily inactivated by traditional MOFs due to the poor compatibility and the limited mass transfer… read more here.
Keywords: bimetal organic; material capsules; glycosyltransferase; fabricating bimetal ... See more keywords
Two Cooperative Glycosyltransferases Are Responsible for the Sugar Diversity of Saquayamycins Isolated from Streptomyces sp. KY 40-1.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00453
Abstract: Glycosyltransferases are key enzymes involved in the biosynthesis of valuable natural products providing an excellent drug-tailoring tool. Herein, we report the identification of two cooperative glycosyltransferases from the sqn gene cluster directing the biosynthesis of… read more here.
Keywords: sugar diversity; two cooperative; gene; glycosyltransferase ... See more keywords