One-pot synthesis of GDP-l-fucose by a four-enzyme cascade expressed in Lactococcus lactis.
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DOI: 10.1016/j.jbiotec.2017.10.009
Abstract: GDP-l-fucose is an l-fucose donor to synthesize fucosylated compounds such as human milk oligosaccharides or Lewis antigen. In this study, we used Lactococcus lactis subsp. cremoris NZ9000 to express 4 enzymes, ManB, ManC, Gmd, and… read more here.
Keywords: lactis; pot synthesis; gmd wcag; one pot ... See more keywords