The role of RNA regulators, quorum sensing and c-di-GMP in bacterial biofilm formation.
Sign Up to like & getrecommendations! Published in 2022 at "FEBS open bio"
DOI: 10.1002/2211-5463.13389
Abstract: Biofilms provide an ecological advantage against many environmental stressors, such as pH and temperature, making it the most common life‐cycle stage for many bacteria. These protective characteristics make eradication of bacterial biofilms challenging. This is… read more here.
Keywords: role; biofilm formation; biofilm; gmp ... See more keywords
Encapsulation of lipases by nucleotide/metal ion coordination polymers: Enzymatic properties and their applications in glycerolysis and esterification studies.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.11749
Abstract: BACKGROUND In this study, lipases of TLL (lipase from Thermomyces lanuginosus), AOL (lipase from Aspergillus oryzae), RML (lipase from Rhizomucor miehei), BCL (lipase from Burkholderia cepacia), CALA (Candida antarctica lipase A) and LU (Lecitase® Ultra)… read more here.
Keywords: rml; cala gmp; glycerolysis; gmp ... See more keywords
Fluorescent 2-Aminopurine c-di-GMP and GpG Analogs as PDE Probes.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7240-1_19
Abstract: c-di-GMP is widely recognized as an important ubiquitous signaling molecule in bacteria. c-di-GMP phosphodiesterases (PDEs) regulate the intracellular concentration of c-di-GMP and some could be potential drug targets. Here, we describe a class of dinucleotide… read more here.
Keywords: gmp gpg; fluorescent aminopurine; gpg analogs; analogs pde ... See more keywords
High-Throughput Screening for Compounds that Modulate the Cellular c-di-GMP Level in Bacteria.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7240-1_33
Abstract: Bacteria in the biofilm mode of growth cause numerous problematic infections due to their resistance to antimicrobials and the immune system. Because conventional antimicrobial compounds cannot efficiently eradicate biofilm infections, we urgently need new efficient… read more here.
Keywords: gmp; high throughput; level; level bacteria ... See more keywords
Gauging and Visualizing c-di-GMP Levels in Pseudomonas aeruginosa Using Fluorescence-Based Biosensors.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7240-1_8
Abstract: Recent research has shown that the molecule c-di-GMP is an important second messenger regulating various functions in bacteria. In particular, the implication of c-di-GMP as a positive regulator of adhesion and biofilm formation has gained… read more here.
Keywords: pseudomonas aeruginosa; gmp levels; fluorescence based; gmp ... See more keywords
Comprehensive characterization of Guanosine monophosphate synthetase in Nicotiana tabacum.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular biology reports"
DOI: 10.1007/s11033-021-06718-x
Abstract: BACKGROUND Guanosine monophosphate (GMP) synthetase is an enzyme that converts xanthosine monophosphate to GMP. GMP plays an essential role in plant development and responses to internal and external stimuli. It also plays a crucial role… read more here.
Keywords: tobacco; synthase; gmp; gmp synthase ... See more keywords
Guanosine and GMP increase the number of granular cerebellar neurons in culture: dependence on adenosine A2A and ionotropic glutamate receptors
Sign Up to like & getrecommendations! Published in 2019 at "Purinergic Signalling"
DOI: 10.1007/s11302-019-09677-y
Abstract: The guanine-based purines (GBPs) have essential extracellular functions such as modulation of glutamatergic transmission and trophic effects on neurons and astrocytes. We previously showed that GBPs, such as guanosine-5′-monophosphate (GMP) or guanosine (GUO), promote the… read more here.
Keywords: cerebellar neurons; gmp; number; glutamate receptors ... See more keywords
NMR resonance assignments for the GSPII-C domain of the PilF ATPase from Thermus thermophilus in complex with c-di-GMP.
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR assignments"
DOI: 10.1007/s12104-019-09906-w
Abstract: The natural transformation system of the thermophilic bacterium Thermus thermophilus is one of the most efficient DNA transport systems in terms of DNA uptake rate and promiscuity. The DNA transporter of T. thermophilus plays an… read more here.
Keywords: atpase; gmp; thermus thermophilus; thermophilus ... See more keywords
NMR resonance assignments for the GSPII-B domain of the traffic ATPase PilF from Thermus thermophilus in the apo and the c-di-GMP-bound state.
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR assignments"
DOI: 10.1007/s12104-019-09911-z
Abstract: The PilF protein from the thermophilic bacterium Thermus thermophilus is a traffic ATPase powering the assembly of the DNA translocation machinery as well as of type 4 pili. Thereby PilF mediates the natural transformability of… read more here.
Keywords: atpase; gmp; pilf; domain ... See more keywords
Towards Maximum Acceleration of Monoclonal Antibody Development: Leveraging Transposase-Mediated Cell Line Generation to Enable GMP Manufacturing within 3 Months using a Stable Pool.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2022.03.010
Abstract: In recent years, acceleration of development timelines has become a major focus within the biopharmaceutical industry to bring innovative therapies faster to patients. However, in order to address a high unmet medical need even faster… read more here.
Keywords: development; cell line; stable pool; gmp ... See more keywords
Construction of an ATMP manufacturing facility and scale up of production: Advent Bioservices
Sign Up to like & getrecommendations! Published in 2020 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2020.03.331
Abstract: Background & Aim In recent years, the UK cell and gene therapy industry has expanded rapidly. Cleanroom space capacity has increased during the last five years to a total of 7,819m2. In 2019, cleanroom facilities… read more here.
Keywords: advent bioservices; production; facility; gmp ... See more keywords