Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0868-5_11
Abstract: Budding yeast Saccharomyces cerevisiae has become a model eukaryotic microorganism for targeted genomic manipulation due to its efficient homologous recombination. A few genomic loci, including rDNA, Delta, and Ty1, can be utilized to introduce variable… read more here.
Keywords: copy; gate assembly; genetic elements; complex genetic ... See more keywords
Golden Gate vectors for efficient gene fusion and gene deletion in diverse filamentous fungi
Sign Up to like & getrecommendations! Published in 2020 at "Current Genetics"
DOI: 10.1007/s00294-020-01143-2
Abstract: The cloning of plasmids can be time-consuming or expensive. Yet, cloning is a prerequisite for many standard experiments for the functional analysis of genes, including the generation of deletion mutants and the localization of gene… read more here.
Keywords: gate vectors; deletion; gene deletion; gene ... See more keywords
A User’s Guide to Golden Gate Cloning Methods and Standards
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00355
Abstract: The continual demand for specialized molecular cloning techniques that suit a broad range of applications has driven the development of many different cloning strategies. One method that has gained significant traction is Golden Gate assembly,… read more here.
Keywords: golden gate; guide golden; cloning methods; user guide ... See more keywords
Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-59745-2
Abstract: Fabs offer an attractive platform for monoclonal antibody discovery/engineering, but library construction can be cumbersome. We report a simple method – Golden Gate assembly with a bi-directional promoter (GBid) – for constructing phage display Fab… read more here.
Keywords: promoter; phage display; gbid; directional promoter ... See more keywords
Efficient golden gate assembly of DNA constructs for single molecule force spectroscopy and imaging
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac300
Abstract: Abstract Single-molecule techniques such as optical tweezers and fluorescence imaging are powerful tools for probing the biophysics of DNA and DNA-protein interactions. The application of these methods requires efficient approaches for creating designed DNA structures… read more here.
Keywords: single molecule; dna constructs; dna; golden gate ... See more keywords
Golden Gate Assembly system dedicated to complex pathway manipulation in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2017 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.12605
Abstract: In this study, we have adopted Golden Gate modular cloning strategy to develop a robust and versatile DNA assembly platform for the nonconventional yeast Yarrowia lipolytica. To this end, a broad set of destination vectors… read more here.
Keywords: system; gate; pathway; golden gate ... See more keywords
Golden Gate Assembly of Aerobic and Anaerobic Microbial Bioreporters.
Sign Up to like & getrecommendations! Published in 2021 at "Applied and environmental microbiology"
DOI: 10.1128/aem.01485-21
Abstract: Microbial bioreporters provide direct insight into cellular processes by producing a quantifiable signal dictated by reporter gene expression. The core of a bioreporter is a genetic circuit in which a reporter gene (or operon) is… read more here.
Keywords: mercury arsenic; biology; microbial bioreporters; gate assembly ... See more keywords
Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0189892
Abstract: Synthetic biology builds upon the foundation of engineering principles, prompting innovation and improvement in biotechnology via a design-build-test-learn cycle. A community-wide standard in DNA assembly would enable bio-molecular engineering at the levels of predictivity and… read more here.
Keywords: mobius assembly; assembly; golden gate; dna assembly ... See more keywords
Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2021-0083
Abstract: Aims: New methods of DNA recombination that capture the principal advantages of the BioBrick standard (ease of design) and Golden Gate assembly (decreased labor) are demonstrated here. Methods & materials: Both methods employ DNA methyltransferase… read more here.
Keywords: golden gate; restriction endonucleases; gate assembly; assembly ... See more keywords
Easy method for six-fragment Golden Gate Assembly of modular vectors.
Sign Up to like & getrecommendations! Published in 2023 at "BioTechniques"
DOI: 10.2144/btn-2022-0041
Abstract: Efficient cloning techniques are a requirement for synthetic biology. This study provides a simplified cloning method based on Golden Gate Assembly that can be used for rapid vector construction. The building of multiple expression vectors… read more here.
Keywords: easy method; golden gate; gate assembly; method six ... See more keywords
A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2018.03345
Abstract: We present a Bacterial Expression Vector Archive (BEVA) for the modular assembly of bacterial vectors compatible with both traditional and Golden Gate cloning, utilizing the Type IIS restriction enzyme Esp3I, and have demonstrated its use… read more here.
Keywords: vector; golden gate; expression vector; bacterial expression ... See more keywords