i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases
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DOI: 10.1186/s13059-018-1400-x
Abstract: We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation. The method generates mouse models containing single-base changes, kilobase-sized deletions,… read more here.
Keywords: gonad robust; robust method; method; method situ ... See more keywords