Co-IP assays for measuring GPCR-arrestin interactions.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.09.003
Abstract: βarrestin1 and -2 (also known as arrestin2 and -3, respectively) are G protein-coupled receptor (GPCR) adapter proteins, performing three major functions in the cell: functional desensitization, i.e., G protein uncoupling from the receptor, GPCR internalization… read more here.
Keywords: gpcr arrestin; gpcr; measuring gpcr; assays measuring ... See more keywords
Improved Split TEV GPCR β-arrestin-2 Recruitment Assays via Systematic Analysis of Signal Peptide and β-arrestin Binding Motif Variants
Sign Up to like & getrecommendations! Published in 2022 at "Biosensors"
DOI: 10.3390/bios13010048
Abstract: G protein-coupled receptors (GPCRs) are major disease-relevant drug targets; robust monitoring of their activities upon drug treatment is key to drug discovery. The split TEV cell-based assay technique monitors the interaction of an activated GPCR… read more here.
Keywords: gpcr arrestin; split tev; arrestin recruitment; tev ... See more keywords