Articles with "granulosa cells" as a keyword



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Downregulation of gap junctional intercellular communication and connexin 43 expression by bisphenol A in human granulosa cells

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Published in 2020 at "Biotechnology and Applied Biochemistry"

DOI: 10.1002/bab.1979

Abstract: Gap junctional intercellular communication (GJIC) is the transfer of ions, metabolites, and second messengers between neighboring cells through intercellular junctions. Connexin 43 (Cx43) was found to be the type of gap junction protein responsible for… read more here.

Keywords: granulosa cells; communication; junctional intercellular; intercellular communication ... See more keywords
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Three‐dimensional culture of rat ovarian granulosa cells shows increased SPP1 and FGF7 expression through the PI3K/Akt pathway

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Published in 2023 at "Cell Biology International"

DOI: 10.1002/cbin.11998

Abstract: Ovarian granulosa cells (OGCs) play an essential role in the regulation of follicular growth and development. However, previous studies of OGCs have concentrated on traditional 2D cultures. In the present study, we used the hanging… read more here.

Keywords: culture; pi3k akt; ovarian granulosa; granulosa cells ... See more keywords
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Ameliorative potential of vitamin C and E against Roundup‐glyphosate induced genotoxicity triggering apoptosis in caprine granulosa cells

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Published in 2022 at "Environmental and Molecular Mutagenesis"

DOI: 10.1002/em.22497

Abstract: The present study was aimed to investigate the genotoxic and apoptotic effects of glyphosate (GLP) in Roundup formulation along with mitigation of two potent antioxidants that is, vitamin C and E in caprine granulosa cells… read more here.

Keywords: induced genotoxicity; roundup; caprine granulosa; granulosa ... See more keywords
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Prenatal exposure to bisphenol‐A altered miRNA‐224 and protein expression of aromatase in ovarian granulosa cells concomitant with elevated serum estradiol levels in F1 adult offspring

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Published in 2019 at "Journal of Biochemical and Molecular Toxicology"

DOI: 10.1002/jbt.22317

Abstract: This study was aimed to predict bisphenol‐A (BPA)‐responsive miRNA's using an in silico approach and to study their expression in granulosa cells of animals exposed prenatally to BPA. Pregnant Wistar rats were exposed to BPA… read more here.

Keywords: ovarian granulosa; granulosa cells; elevated serum; mirna 224 ... See more keywords
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3,3′,4,4′,5‐Pentachlorobiphenyl influences mitochondrial apoptosis pathway in granulosa cells

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Published in 2019 at "Journal of Cellular Biochemistry"

DOI: 10.1002/jcb.28801

Abstract: 3,3′,4,4′,5‐Polychlorinated biphenyl (PCB126) is a persistent organic environmental pollutant which can affect various biological activities of organisms, such as immunity, neurological function, and reproduction. In our study, we aimed to investigate the effects of PCB126… read more here.

Keywords: granulosa cells; mitochondrial apoptosis; apoptosis; gcs ... See more keywords
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Effect of mono‐(2‐ethylhexyl) phthalate (MEHP) on proliferation of and steroid hormone synthesis in rat ovarian granulosa cells in vitro

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Published in 2018 at "Journal of Cellular Physiology"

DOI: 10.1002/jcp.26224

Abstract: This study aimed to examine the proliferation of and secretion by rat ovarian granulosa cells (GCs) treated with mono‐(2‐ethylhexyl) phthalate (MEHP). Ovarian GCs were incubated with MEHP at concentration of 0, 25, 50, 100, and… read more here.

Keywords: hormone; granulosa cells; ovarian granulosa; mono ethylhexyl ... See more keywords
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Arachidonic acid alleviates the detrimental effects of acetylsalicylic acid on human granulosa cells performance in vitro

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Published in 2020 at "Molecular Reproduction and Development"

DOI: 10.1002/mrd.23343

Abstract: Here, we investigated the biological effects of arachidonic acid (AA) in human cumulus granulosa cells (CGCs) after exposure to ASA. Cells were isolated from the follicular fluid and incubated with 0.5 mM acetylsalicylic acid (ASA) and… read more here.

Keywords: granulosa cells; acid human; acetylsalicylic acid; arachidonic acid ... See more keywords
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Interaction between growing oocytes and granulosa cells in vitro

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Published in 2020 at "Reproductive Medicine and Biology"

DOI: 10.1002/rmb2.12292

Abstract: Oocyte growth is accompanied by follicular development in mammalian ovaries. Since the discovery of two oocyte‐derived factors, growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15), knowledge of the bidirectional communication between oocytes… read more here.

Keywords: granulosa cells; oocytes granulosa; growing oocytes; interaction growing ... See more keywords
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Ovine Granulosa Cells Isolation and Culture to Improve Oocyte Quality.

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Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-8600-2_10

Abstract: The functional cooperation between the oocyte and its surrounding granulosa cells is mandatory to oocyte growth and acquisition of developmental competence in mammalian species. Availability of in vitro methods for culture granulosa cells is important… read more here.

Keywords: granulosa cells; ovine granulosa; biology; improve oocyte ... See more keywords
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Bioencapsulation of Oocytes and Granulosa Cells.

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Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-8600-2_9

Abstract: A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of… read more here.

Keywords: bioencapsulation oocytes; granulosa cells; oocytes granulosa; biology ... See more keywords
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Circular RNA expression profiling of granulosa cells in women of reproductive age with polycystic ovary syndrome

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Published in 2019 at "Archives of Gynecology and Obstetrics"

DOI: 10.1007/s00404-019-05129-5

Abstract: PurposeThis study aimed to explore the expression profiles of circRNA in granulosa cells of women of reproductive age with polycystic ovary syndrome (PCOS).MethodsTotal RNA was isolated from granulosa cells of 15 women with PCOS and… read more here.

Keywords: granulosa cells; cells women; age; women reproductive ... See more keywords