One‐step generation of mice with gene editing by Tol2 transposon‐dependent gRNA delivery
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14605
Abstract: Conditional knockout mice are valuable tools for examining the functions of targeted genes in a time‐ and space‐specific manner. Here, we generated gene‐edited mice by using the Tol2 transposon to introduce guide RNA (gRNA) into… read more here.
Keywords: tol2 transposon; grna; one step; step generation ... See more keywords
The yeast platform engineered for synthetic gRNA-landing pads enables multiple gene integrations by a single gRNA/Cas9 system.
Sign Up to like & getrecommendations! Published in 2021 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2021.01.011
Abstract: Saccharomyces cerevisiae is a versatile microbial platform to build synthetic metabolic pathways for production of diverse chemicals. To expedite the construction of complex metabolic pathways by multiplex CRISPR-Cas9 genome-edit, eight desirable intergenic loci, located adjacent… read more here.
Keywords: platform; gene integrations; synthetic grna; grna ... See more keywords
Efficient Genome Editing Achieved via Plug-and-Play Adenovirus Piggyback Transport of Cas9/gRNA Complex on Viral Capsid Surface
Sign Up to like & getrecommendations! Published in 2022 at "ACS Nano"
DOI: 10.1021/acsnano.2c00909
Abstract: The capacity to efficiently deliver the gene-editing enzyme complex to target cells is favored over other forms of gene delivery as it offers one-time hit-and-run gene editing, thus improving precision and safety and reducing potential… read more here.
Keywords: grna; gene editing; grna complex; surface ... See more keywords
An overview and metanalysis of machine and deep learning-based CRISPR gRNA design tools
Sign Up to like & getrecommendations! Published in 2019 at "RNA Biology"
DOI: 10.1080/15476286.2019.1669406
Abstract: ABSTRACT The CRISPR-Cas9 system has become the most promising and versatile tool for genetic manipulation applications. Albeit the technology has been broadly adopted by both academic and pharmaceutic societies, the activity (on-target) and specificity (off-target)… read more here.
Keywords: crispr; crispr grna; deep learning; machine deep ... See more keywords
Evaluation of efficiency prediction algorithms and development of ensemble model for CRISPR/Cas9 gRNA selection
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac681
Abstract: MOTIVATION The CRISPR/Cas9 system is widely used for genome editing. The editing efficiency of CRISPR/Cas9 is mainly determined by the guide RNA (gRNA). Although many computational algorithms have been developed in recent years, it is… read more here.
Keywords: crispr cas9; algorithms; crispr; grna ... See more keywords
Generating minimum set of gRNA to cover multiple targets in multiple genomes with MINORg
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad142
Abstract: MINORg is an offline gRNA design tool that generates the smallest possible combination of gRNA capable of covering all desired targets in multiple non-reference genomes. As interest in pangenomic research grows, so does the workload… read more here.
Keywords: targets multiple; generating minimum; minimum set; grna ... See more keywords
Trypanosome RNA helicase KREH2 differentially controls non-canonical editing and putative repressive structure via a novel proposed 'bifunctional' gRNA in mRNA A6.
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic acids research"
DOI: 10.1093/nar/gkad453
Abstract: U-insertion/deletion (U-indel) RNA editing in trypanosome mitochondria is directed by guide RNAs (gRNAs). This editing may developmentally control respiration in bloodstream forms (BSF) and insect procyclic forms (PCF). Holo-editosomes include the accessory RNA Editing Substrate… read more here.
Keywords: structure; grna; rna; canonical editing ... See more keywords
Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA
Sign Up to like & getrecommendations! Published in 2022 at "Plant Physiology"
DOI: 10.1093/plphys/kiac128
Abstract: Abstract As the focus for CRISPR/Cas-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing for polygenic traits. A common approach for editing across multiple sites is to… read more here.
Keywords: grna; multiplex knockout; hybrid poplar; single grna ... See more keywords
Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies
Sign Up to like & getrecommendations! Published in 2017 at "Microbial Cell Factories"
DOI: 10.1186/s12934-017-0664-2
Abstract: BackgroundTranscriptional reprogramming is a fundamental process of living cells in order to adapt to environmental and endogenous cues. In order to allow flexible and timely control over gene expression without the interference of native gene… read more here.
Keywords: target; expression; yeast; transcriptional reprogramming ... See more keywords
Translation of SARS-CoV-2 gRNA Is Extremely Efficient and Competitive despite a High Degree of Secondary Structures and the Presence of an uORF
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14071505
Abstract: The SARS-CoV-2 infection generates up to nine different sub-genomic mRNAs (sgRNAs), in addition to the genomic RNA (gRNA). The 5′UTR of each viral mRNA shares the first 75 nucleotides (nt.) at their 5′end, called the… read more here.
Keywords: grna; presence; cov grna; sars cov ... See more keywords