Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction
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DOI: 10.1186/s12864-025-11317-2
Abstract: Ensuring sufficient gRNA transcript levels is critical for obtaining optimal CRISPR-Cas9 gene editing efficiency. The standard gRNA scaffold contains a sequence of four thymine nucleotides (4T), which is known to inhibit transcription from Pol III… read more here.
Keywords: transcript; editing efficiency; transcript levels; grna transcript ... See more keywords