Therapeutic applications of group I intron‐based trans‐splicing ribozymes
Sign Up to like & getrecommendations! Published in 2018 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1466
Abstract: Since the breakthrough discovery of catalytic RNAs (ribozymes) in the early 1980s, valuable ribozyme‐based gene therapies have been developed for incurable diseases ranging from genetic disorders to viral infections and cancers. Ribozymes can be engineered… read more here.
Keywords: splicing ribozymes; group intron; gene; trans splicing ... See more keywords
Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9904-0_7
Abstract: Chimeric RNAs are the transcripts composed of exons from two separate genes or transcripts. Although the presence of these joined RNA molecules have mainly been documented in a variety of eukaryotes, we recently demonstrated that… read more here.
Keywords: detection group; group; group intron; intron generated ... See more keywords
Influence of pH and Mg(ii) on the catalytic core domain 5 of a bacterial group II intron.
Sign Up to like & getrecommendations! Published in 2017 at "Dalton transactions"
DOI: 10.1039/c6dt04784j
Abstract: RNA molecules fold into complex structures that allow them to perform specific functions. To compensate the relative lack of diversity of functional groups within nucleotides, metal ions work as crucial co-factors. In addition, shifted pKas… read more here.
Keywords: group intron; domain; spectroscopy; influence catalytic ... See more keywords
Direct tracking of reverse-transcriptase speed and template sensitivity: implications for sequencing and analysis of long RNA molecules
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac518
Abstract: Abstract Although reverse-transcriptase (RT) enzymes are critical reagents for research and biotechnology, their mechanical properties are not well understood. In particular, we know little about their relative speed and response to structural obstacles in the… read more here.
Keywords: speed; group intron; reverse transcriptase; direct tracking ... See more keywords
Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx867
Abstract: Abstract Cellular accumulation of repetitive RNA occurs in several dominantly-inherited genetic disorders. Expanded CUG, CCUG or GGGGCC repeats are expressed in myotonic dystrophy type 1 (DM1), myotonic dystrophy type 2 (DM2), or familial amyotrophic lateral… read more here.
Keywords: intron reverse; rna; reverse; group intron ... See more keywords
Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.834020
Abstract: RmInt1 is a group II intron encoding a reverse transcriptase protein (IEP) lacking the C-terminal endonuclease domain. RmInt1 is an efficient mobile retroelement that predominantly reverse splices into the transient single-stranded DNA at the template… read more here.
Keywords: group intron; dna; replication; group ... See more keywords
Group I Intron as a Potential Target for Antifungal Compounds: Development of a Trans-Splicing High-Throughput Screening Strategy
Sign Up to like & getrecommendations! Published in 2023 at "Molecules"
DOI: 10.3390/molecules28114460
Abstract: The search for safe and efficient new antifungal compounds for agriculture has led to more efforts in finding new modes of action. This involves the discovery of new molecular targets, including coding and non-coding RNA.… read more here.
Keywords: group intron; antifungal compounds; throughput screening; group ... See more keywords