Systematizing the different notions of growth-coupled product synthesis and a single framework for computing corresponding strain designs.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology journal"
DOI: 10.1002/biot.202100236
Abstract: BACKGROUND A widely used design principle for metabolic engineering of microorganisms aims to introduce interventions that enforce growth-coupled product synthesis such that the product of interest becomes a (mandatory) by-product of growth. However, different variants… read more here.
Keywords: growth coupled; product; product synthesis; strain designs ... See more keywords
Growth-coupled evolution and high-throughput screening assisted rapid enhancement for amylase-producing Bacillus licheniformis.
Sign Up to like & getrecommendations! Published in 2021 at "Bioresource technology"
DOI: 10.1016/j.biortech.2021.125467
Abstract: Bacillus licheniformis α-amylase is a thermostable enzyme used in industrial starch hydrolysis. However, difficulties in the genetic manipulation of B. licheniformis hamper further enhancement of α-amylase production. In this regard, adaptive evolution is a useful… read more here.
Keywords: evolution; bacillus licheniformis; growth coupled; amylase ... See more keywords
Engineering Carboxylic Acid Reductase (CAR) through A Whole-Cell Growth-Coupled NADPH Recycling Strategy.
Sign Up to like & getrecommendations! Published in 2020 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.0c00290
Abstract: Rapid evolution of enzyme activities is often hindered by the lack of efficient and affordable methods to identify beneficial mutants. We report the development of a new growth-coupled selection method for evolving NADPH-consuming enzymes based… read more here.
Keywords: reductase car; whole cell; growth coupled; acid reductase ... See more keywords
Suppressed cosmic growth in coupled vector-tensor theories
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DOI: 10.1103/physrevd.102.063531
Abstract: We study a coupled dark energy scenario in which a massive vector field $A_{\mu}$ with broken $U(1)$ gauge symmetry interacts with the four-velocity $u_c^{\mu}$ of cold dark matter (CDM) through the scalar product $Z=-u_c^{\mu} A_{\mu}$.… read more here.
Keywords: growth; growth coupled; cdm; cosmic growth ... See more keywords