High-level extracellular production of Rhizopus oryzae lipase in Pichia pastoris via a strategy combining optimization of gene-copy number with co-expression of ERAD-related proteins.
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DOI: 10.1016/j.pep.2018.02.005
Abstract: Rhizopus oryzae lipase (ROL) is an important industrial enzyme limited in application due to its low production in native strains. Here, we used a new combined strategy to overexpress ROL in Pichia pastoris. An efficient… read more here.
Keywords: production; pastoris; gs115; expression ... See more keywords