Live-Cell CRISPR Imaging in Plant Cells with a Telomere-Specific Guide RNA.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0712-1_20
Abstract: Chromatin organization is highly dynamic in living cells. Therefore, it might have a regulatory role over biological mechanisms like transcription, replication, and DNA repair. To elucidate how these mechanisms are regulated, it is required to… read more here.
Keywords: specific guide; guide rna; plant; telomere specific ... See more keywords
Guide RNA modification as a way to improve CRISPR/Cas9-based genome-editing systems.
Sign Up to like & getrecommendations! Published in 2019 at "Biochimie"
DOI: 10.1016/j.biochi.2019.09.003
Abstract: Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and… read more here.
Keywords: genome editing; guide rna; crispr; crispr cas9 ... See more keywords
Improving CRISPR Genome Editing by Engineering Guide RNAs.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2019.01.009
Abstract: CRISPR technology is a two-component gene editing system in which the effector protein induces genetic alterations with the aid of a gene targeting guide RNA. Guide RNA can be produced through chemical synthesis, in vitro… read more here.
Keywords: guide rnas; genome editing; guide rna; guide ... See more keywords
High-Density Guide RNA Tiling and Machine Learning for Designing CRISPR Interference in Synechococcus sp. PCC 7002.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00653
Abstract: While CRISPRi was previously established in Synechococcus sp. PCC 7002 (hereafter 7002), the design principles for guide RNA (gRNA) effectiveness remain largely unknown. Here, 76 strains of 7002 were constructed with gRNAs targeting three reporter… read more here.
Keywords: guide rna; machine; machine learning; pcc 7002 ... See more keywords
5S rRNA Promoter for Guide RNA Expression Enabled Highly Efficient CRISPR/Cas9 Genome Editing in Aspergillus niger.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.7b00456
Abstract: The CRISPR/Cas9 system is a revolutionary genome editing tool. However, in eukaryotes, search and optimization of a suitable promoter for guide RNA expression is a significant technical challenge. Here we used the industrially important fungus,… read more here.
Keywords: crispr cas9; guide rna; system; promoter ... See more keywords
T7 Polymerase Expression of Guide RNAs in vivo Allows Exportable CRISPR-Cas9 Editing in Multiple Yeast Hosts.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.7b00461
Abstract: Efficient guide RNA expression often limits CRISPR-Cas9 implementation in new hosts. To address this limitation in fungal systems, we demonstrate the utility of a T7 polymerase system to effectively express sgRNAs. Initially, we developed a… read more here.
Keywords: guide rna; crispr cas9; polymerase expression; guide ... See more keywords
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15892-8
Abstract: CRISPR-Cas9-associated base editing is a promising tool to correct pathogenic single nucleotide mutations in research or therapeutic settings. Efficient base editing requires cellular exposure to levels of base editors that can be difficult to attain… read more here.
Keywords: guide rna; editor; base; base editor ... See more keywords
Theophylline-induced synergic activation of guide RNA to control CRISPR/Cas9 function.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical communications"
DOI: 10.1039/d1cc01260f
Abstract: Ligand-induced activation of CRISPR/Cas9 function is achieved based on a synergic approach through the integration of the theophylline aptamer into protein-unrecognized regions of guide RNA. This design of allosteric regulation opens a new window towards… read more here.
Keywords: crispr cas9; guide rna; cas9 function;
Regulation of CRISPR-Cas12a system by methylation and demethylation of Guide RNA
Sign Up to like & getrecommendations! Published in 2023 at "Chemical Science"
DOI: 10.1039/d3sc00629h
Abstract: Chemical modifications of CRISPR-Cas nucleases help decrease off-target editing and expand the biomedical applications of CRISPR-based gene manipulation tools. Here, we found that epigenetic modifications of guide RNA, such as m6A and m1A methylation, can… read more here.
Keywords: crispr cas12a; regulation crispr; methylation; guide rna ... See more keywords
The hitchhikers' guide to RNA sequencing and functional analysis
Sign Up to like & getrecommendations! Published in 2023 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac529
Abstract: DNA and RNA sequencing technologies have revolutionized biology and biomedical sciences, sequencing full genomes and transcriptomes at very high speeds and reasonably low costs. RNA sequencing (RNA-Seq) enables transcript identification and quantification, but once sequencing… read more here.
Keywords: rna sequencing; guide rna; sequencing functional; rna seq ... See more keywords
Enhanced guide-RNA design and targeting analysis for precise CRISPR genome editing of single and consortia of industrially relevant and non-model organisms
Sign Up to like & getrecommendations! Published in 2018 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btx564
Abstract: Motivation Genetic diversity of non-model organisms offers a repertoire of unique phenotypic features for exploration and cultivation for synthetic biology and metabolic engineering applications. To realize this enormous potential, it is critical to have an… read more here.
Keywords: genome editing; guide rna; crispr; analysis ... See more keywords