Chemical Control of CRISPR Gene Editing via Conditional Diacylation Crosslinking of Guide RNAs
Sign Up to like & getrecommendations! Published in 2023 at "Advanced Science"
DOI: 10.1002/advs.202206433
Abstract: Conditional control of RNA structure and function has emerged as an effective toolkit. Here, a strategy based on a one‐step introduction of diacylation linkers and azide groups on the 2′‐OH of RNA is advance. Selected… read more here.
Keywords: gene editing; chemical control; guide rnas; diacylation ... See more keywords
Chemical Modification and Transformation Strategies of Guide RNAs in CRISPR-Cas9 Gene Editing Systems.
Sign Up to like & getrecommendations! Published in 2021 at "ChemPlusChem"
DOI: 10.1002/cplu.202000785
Abstract: The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated protein 9) is a most powerful tool and has been widely used in gene editing and gene regulation since its discovery. However, wild-type CRISPR-Cas9 suffers from… read more here.
Keywords: guide rnas; gene editing; crispr cas9; gene ... See more keywords
Cas9 deactivation with photocleavable guide RNAs.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular cell"
DOI: 10.1016/j.molcel.2021.02.007
Abstract: Precise control of CRISPR-Cas9 would improve its safety and applicability. Controlled CRISPR inhibition is a promising approach but is complicated by separate inhibitor delivery, incomplete deactivation, and slow kinetics. To overcome these obstacles, we engineered… read more here.
Keywords: guide rnas; deactivation; cas9 deactivation; photocleavable guide ... See more keywords
Improving CRISPR Genome Editing by Engineering Guide RNAs.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2019.01.009
Abstract: CRISPR technology is a two-component gene editing system in which the effector protein induces genetic alterations with the aid of a gene targeting guide RNA. Guide RNA can be produced through chemical synthesis, in vitro… read more here.
Keywords: guide rnas; genome editing; guide rna; guide ... See more keywords
Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona.
Sign Up to like & getrecommendations! Published in 2017 at "Developmental biology"
DOI: 10.1016/j.ydbio.2017.03.003
Abstract: The CRISPR/Cas9 system has emerged as an important tool for various genome engineering applications. A current obstacle to high throughput applications of CRISPR/Cas9 is the imprecise prediction of highly active single guide RNAs (sgRNAs). We… read more here.
Keywords: crispr cas9; guide rnas; crispr; mutagenesis ... See more keywords
On-line Sequencing of CRISPR Guide RNAs and Their Impurities via the Use of Immobilized Ribonuclease Cartridges Attached to a 2D/3D-LC-MS System.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04350
Abstract: In this study, for the first time, the automated digestion and sequencing of an RNA molecule via the use of immobilized RNase cartridges attached to a multidimensional liquid chromatography (LC)-mass spectrometry (MS) system are presented.… read more here.
Keywords: use immobilized; via use; line; cartridges attached ... See more keywords
Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 Editors.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.1c00768
Abstract: CRISPR gene editing and control systems continue to emerge and inspire novel research and clinical applications. Advances in CRISPR performance such as optimizing the duration of activity in cells, tissues, and organisms, as well as… read more here.
Keywords: modified ends; modifications enhance; guide rnas; editing yields ... See more keywords
CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17952-5
Abstract: Precise genome editing using CRISPR-Cas9 is a promising therapeutic avenue for genetic diseases, although off-target editing remains a significant safety concern. Guide RNAs shorter than 16 nucleotides in length effectively recruit Cas9 to complementary sites… read more here.
Keywords: crispr guard; guide rnas; target; activity ... See more keywords
PrimeDesign software for rapid and simplified design of prime editing guide RNAs.
Sign Up to like & getrecommendations! Published in 2021 at "Nature communications"
DOI: 10.1038/s41467-021-21337-7
Abstract: Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web… read more here.
Keywords: prime editing; guide rnas; editing guide; design ... See more keywords
Predicting the efficiency of prime editing guide RNAs in human cells
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-020-0677-y
Abstract: Prime editing enables the introduction of virtually any small-sized genetic change without requiring donor DNA or double-strand breaks. However, evaluation of prime editing efficiency requires time-consuming experiments, and the factors that affect efficiency have not… read more here.
Keywords: guide rnas; editing guide; prime editing; efficiency ... See more keywords
Computational design of CRISPR guide RNAs to enable strain-specific control of microbial consortia.
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2213154120
Abstract: Microbes naturally coexist in complex, multistrain communities. However, extracting individual microbes from and specifically manipulating the composition of these consortia remain challenging. The sequence-specific nature of CRISPR guide RNAs can be leveraged to accurately differentiate… read more here.
Keywords: crispr guide; crispr; strain specific; consortia ... See more keywords