Structure-Guided Engineering of CYP109B4: From Monooxygenase to Peroxygenase for Selective Steroid Hydroxylation.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.5c11530
Abstract: Cytochrome P450 CYP109B4 is a characterized 16β-steroid hydroxylase with significant potential in steroid drug biosynthesis, but its application is limited by reliance on costly coenzymes and complex electron transfer processes. To address this, we constructed… read more here.
Keywords: structure guided; guided engineering; cyp109b4; peroxygenase ... See more keywords
Chemoinformatic-guided engineering of polyketide synthases.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.0c02549
Abstract: Polyketide synthase (PKS) engineering is an attractive method to generate new molecules such as commodity, fine and spe-cialty chemicals. A significant challenge in PKS design is engineering a partially reductive module to produce a saturated… read more here.
Keywords: engineering; reductive loops; guided engineering; engineering polyketide ... See more keywords
Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-46239-2
Abstract: The Hydroxycarboxylic acid receptor 2 (HCA2), also known as the niacin receptor or GPR109A, is a prototypical GPCR that plays a central role in the inhibition of lipolytic and atherogenic activities. Its activation also results… read more here.
Keywords: receptor; guided engineering; engineering biased; structure guided ... See more keywords
Structure-guided engineering of tick evasins for targeting chemokines in inflammatory diseases
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2122105119
Abstract: Significance Inflammatory diseases collectively account for numerous deaths and morbidity worldwide. New treatment approaches are needed. A central feature of inflammatory diseases is the recruitment of leukocytes to the affected tissues, which is stimulated by… read more here.
Keywords: structure guided; tick evasins; guided engineering; structure ... See more keywords