Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1597-3_8
Abstract: Sequential ChIP (ChIP-reChIP) enables the characterization of the same nucleosome for two different types of modifications or histone subtypes. Here, we describe a ChIP-reChIP protocol to identify a heterotypic (asymmetric) H2A.Z-H2A-containing nucleosome. In this method,… read more here.
Keywords: heterotypic; h2a; sequential chromatin; identify heterotypic ... See more keywords
An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6630-1_2
Abstract: The incorporation of histone variants into specific chromatin regions is a mechanism by which cells can regulate many important biological processes. One such example is H2A.Z, a highly conserved variant of H2A that is incorporated… read more here.
Keywords: vitro assay; variant exchange; h2a; exchange ... See more keywords
Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome
Sign Up to like & getrecommendations! Published in 2019 at "Cell"
DOI: 10.1016/j.cell.2019.08.002
Abstract: The developmental disorder Floating-Harbor syndrome (FHS) is caused by heterozygous truncating mutations in SRCAP, a gene encoding a chromatin remodeler mediating incorporation of histone variant H2A.Z. Here, we demonstrate that FHS-associated mutations result in loss… read more here.
Keywords: h2a; h2a subtypes; amino acid; human syndrome ... See more keywords
Maintenance of meiotic crossover against reduced double-strand break formation in fission yeast lacking histone H2A.Z.
Sign Up to like & getrecommendations! Published in 2020 at "Gene"
DOI: 10.1016/j.gene.2020.144615
Abstract: Meiotic crossover (CO) recombination initiates from programmed DNA double-strand breaks (DSBs) around hotspots, and results in reciprocal exchange of chromosome segments between homologous chromosomes (homologs). COs are crucial for most sexually-reproducing organisms because it promotes… read more here.
Keywords: formation; histone h2a; h2a; double strand ... See more keywords
Neutrophil extracellular traps: The synergy source in the placentae of HIV infected women with pre-eclampsia.
Sign Up to like & getrecommendations! Published in 2020 at "Pregnancy hypertension"
DOI: 10.1016/j.preghy.2020.03.007
Abstract: OBJECTIVES To immuno-localize histone H2A expression as a marker of neutrophil extracellular traps (NETs) in the placenta; and to quantify and compare the percentage H2A immune-expression as a marker of NETs in the placental intervillous… read more here.
Keywords: neutrophil extracellular; hiv; group; h2a ... See more keywords
High-Speed Atomic Force Microscopy Reveals Spontaneous Nucleosome Sliding of H2A.Z at the Subsecond Time Scale.
Sign Up to like & getrecommendations! Published in 2023 at "Nano letters"
DOI: 10.1021/acs.nanolett.2c04346
Abstract: Nucleosome dynamics, such as nucleosome sliding and DNA unwrapping, are important for gene regulation in eukaryotic chromatin. H2A.Z, a variant of histone H2A that is highly evolutionarily conserved, participates in gene regulation by forming unstable… read more here.
Keywords: high speed; speed atomic; microscopy; nucleosome sliding ... See more keywords
Histone H2A Ubiquitination Reinforces Mechanical Stability and Asymmetry at Single-nucleosome Level.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b12448
Abstract: Mono-ubiquitination at Lysine 119 of H2A (ubH2A) is a prevalent post-translational modification and associated with gene repression in the context of chromatin. However, the direct function of ubH2A on nucleosome is poorly understood. Here we… read more here.
Keywords: histone h2a; asymmetry; h2a; ubh2a nucleosome ... See more keywords
Histone variant H2A.J accumulates in senescent cells and promotes inflammatory gene expression
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms14995
Abstract: The senescence of mammalian cells is characterized by a proliferative arrest in response to stress and the expression of an inflammatory phenotype. Here we show that histone H2A.J, a poorly studied H2A variant found only… read more here.
Keywords: variant h2a; senescent cells; h2a; expression ... See more keywords
Structural basis of specific H2A K13/K15 ubiquitination by RNF168
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-09756-z
Abstract: Ubiquitination of chromatin by modification of histone H2A is a critical step in both regulation of DNA repair and regulation of cell fate. These very different outcomes depend on the selective modification of distinct lysine… read more here.
Keywords: basis; h2a; ubiquitination; k13 k15 ... See more keywords
The H2A.Z histone variant integrates Wnt signaling in intestinal epithelial homeostasis
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-09899-z
Abstract: The Tip60/p400 chromatin-modifying complex, which is involved in the incorporation and post-translational modification of the H2A.Z histone variant, regulates cell proliferation and important signaling pathways, such as Wnt. Here, we study the involvement of H2A.Z… read more here.
Keywords: homeostasis; intestinal epithelial; histone variant; h2a ... See more keywords
Effects of charge-modifying mutations in histone H2A α3-domain on nucleosome stability assessed by single-pair FRET and MD simulations
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-13416-x
Abstract: Nucleosomes are important for chromatin compaction and gene regulation; their integrity depends crucially on the structural properties of the histone tails. Recent all-atom molecular dynamics simulations revealed that removal of the N-terminal tails of histone… read more here.
Keywords: charge modifying; histone h2a; nucleosome stability; h2a ... See more keywords