γ‐H2AX formation in the urinary bladder of rats treated with two norharman derivatives obtained from o‐toluidine and aniline
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Applied Toxicology"
DOI: 10.1002/jat.3560
Abstract: Aminomethylphenylnorharman (AMPNH) and aminophenylnorharman (APNH) are mutagenic norharman derivatives obtained from o‐toluidine and aniline, respectively. APNH is carcinogenic to the urinary bladder of rats and present in urine samples of healthy volunteers, indicating that norharman… read more here.
Keywords: bladder rats; h2ax; bladder; formation ... See more keywords
Immunofluorescence Analysis of γ-H2AX Foci in Mammalian Fibroblasts at Different Phases of the Cell Cycle.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7187-9_17
Abstract: H2AX phosphorylation at Ser139 (formation of γ-H2AX) is an indicator of double-strand breaks in DNA (DSBs) after the action of different genotoxic stresses, including ionizing radiation, environmental agents, and chemotherapy drugs. The sites of DSBs… read more here.
Keywords: h2ax foci; microscopy; h2ax; different phases ... See more keywords
2,4-Dimethylaniline generates phosphorylated histone H2AX in human urothelial and hepatic cells through reactive oxygen species produced by cytochrome P450 2E1
Sign Up to like & getrecommendations! Published in 2018 at "Archives of Toxicology"
DOI: 10.1007/s00204-018-2289-6
Abstract: The Japanese Ministry of Health, Labour, and Welfare recently reported an outbreak of bladder cancer among workers who handled aromatic amines in Japan. 2,4-dimethylaniline (2,4-DMA) is one of the chemicals that workers are considered to… read more here.
Keywords: reactive oxygen; histone h2ax; oxygen species; h2ax ... See more keywords
Validation of the γH2AX biomarker for genotoxicity assessment: a review
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Toxicology"
DOI: 10.1007/s00204-019-02511-9
Abstract: The H2AX histone protein is rapidly phosphorylated at the serine-139 position (γH2AX) in response to a broad range of DNA lesions. γH2AX induction is one of the earliest events in the DNA damage response (DDR)… read more here.
Keywords: h2ax biomarker; genotoxicity assessment; h2ax; genotoxicity ... See more keywords
DNA damage in lymphocytes of patients suffering from complex traumatization.
Sign Up to like & getrecommendations! Published in 2017 at "DNA repair"
DOI: 10.1016/j.dnarep.2017.02.006
Abstract: It has been shown that complex childhood traumatization (CCT) increases the risk for severe somatic and mental disorders. However, the somatic pathways linking maladaptive affect regulation and disease are not understood. Here we tested the… read more here.
Keywords: dna damage; traumatization; dna; h2ax ... See more keywords
Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro γH2AX assay using high content screening.
Sign Up to like & getrecommendations! Published in 2017 at "Environmental toxicology and pharmacology"
DOI: 10.1016/j.etap.2017.07.005
Abstract: To evaluate the genotoxic effects of formaldehyde, acetaldehyde, acrolein and benzene on A549 cells, the in vitro γH2AX assay was used in combination with high content screening (HCS) technology. All aldehydes showed a significant genotoxicity… read more here.
Keywords: vitro h2ax; h2ax; h2ax assay; genotoxicity ... See more keywords
Monoubiquitinated &ggr;‐H2AX: Abundant product and specific biomarker for non‐apoptotic DNA double‐strand breaks
Sign Up to like & getrecommendations! Published in 2018 at "Toxicology and Applied Pharmacology"
DOI: 10.1016/j.taap.2018.07.007
Abstract: ABSTRACT DNA double‐strand breaks (DSBs) are a highly toxic form of DNA damage produced by a number of carcinogens, drugs, and metabolic abnormalities. Involvement of DSBs in many pathologies has led to frequent measurements of… read more here.
Keywords: h2ax; h2ax ub1; ggr h2ax; dna double ... See more keywords
Confined Target-Triggered Hot Spots for In Situ SERS Analysis of Intranuclear Genotoxic Markers.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05147
Abstract: The γH2AX is a type of confined target in nuclei which is highly expressed around the damaged DNA during genotoxicity and has therefore been identified as a marker of genotoxicity. Convenient and intuitive in situ… read more here.
Keywords: analysis intranuclear; confined target; hot spots; h2ax ... See more keywords
A High-Throughput Platform for the Rapid Quantification of Phosphorylated Histone H2AX in Cell Lysates Based on Microplate Electrochemiluminescence Immunosensor Array.
Sign Up to like & getrecommendations! Published in 2021 at "ACS sensors"
DOI: 10.1021/acssensors.1c01502
Abstract: Sensitive detection of phosphorylated histone H2AX (γH2AX) in cells as a biomarker of DNA double-strand breaks has great significance in the field of molecular toxicology and life science research. However, current γH2AX detection methods require… read more here.
Keywords: array; cell lysates; h2ax; high throughput ... See more keywords
Identification of the elementary structural units of the DNA damage response
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms15760
Abstract: Histone H2AX phosphorylation is an early signalling event triggered by DNA double-strand breaks (DSBs). To elucidate the elementary units of phospho-H2AX-labelled chromatin, we integrate super-resolution microscopy of phospho-H2AX during DNA repair in human cells with… read more here.
Keywords: h2ax; identification elementary; elementary structural; phospho h2ax ... See more keywords
MDC1 PST-repeat region promotes histone H2AX-independent chromatin association and DNA damage tolerance
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-12929-5
Abstract: Histone H2AX and MDC1 are key DNA repair and DNA-damage signalling proteins. When DNA double-strand breaks (DSBs) occur, H2AX is phosphorylated and then recruits MDC1, which in turn serves as a docking platform to promote… read more here.
Keywords: dna damage; pst repeat; h2ax; mdc1 ... See more keywords