Immunofluorescence Analysis of γ-H2AX Foci in Mammalian Fibroblasts at Different Phases of the Cell Cycle.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7187-9_17
Abstract: H2AX phosphorylation at Ser139 (formation of γ-H2AX) is an indicator of double-strand breaks in DNA (DSBs) after the action of different genotoxic stresses, including ionizing radiation, environmental agents, and chemotherapy drugs. The sites of DSBs… read more here.
Keywords: h2ax foci; microscopy; h2ax; different phases ... See more keywords
A deep learning model (FociRad) for automated detection of γ-H2AX foci and radiation dose estimation.
Sign Up to like & getrecommendations! Published in 2022 at "Scientific reports"
DOI: 10.1038/s41598-022-09180-2
Abstract: DNA double-strand breaks (DSBs) are the most lethal form of damage to cells from irradiation. γ-H2AX (phosphorylated form of H2AX histone variant) has become one of the most reliable and sensitive biomarkers of DNA DSBs.… read more here.
Keywords: h2ax foci; deep learning; h2ax; detection ... See more keywords
Evaluation of γ-H2AX foci distribution among different peripheral blood mononucleated cell subtypes.
Sign Up to like & getrecommendations! Published in 2023 at "International journal of radiation biology"
DOI: 10.1080/09553002.2023.2187480
Abstract: INTRODUCTION The detection of γ-H2AX foci in peripheral blood mononucleated cells (PBMCs) has been incorporated as an early assay for biological dosimetry. However, overdispersion in the γ-H2AX foci distribution is generally reported. In a previous… read more here.
Keywords: cell; cell subtypes; foci distribution; h2ax foci ... See more keywords
High-content imaging analyses of γH2AX-foci and micronuclei in TK6 cells elucidated genotoxicity of chemicals and their clastogenic/aneugenic mode of action
Sign Up to like & getrecommendations! Published in 2019 at "Genes and Environment"
DOI: 10.1186/s41021-019-0117-8
Abstract: BackgroundThe in vitro micronucleus (MN) test is an important component of a genotoxicity test battery that evaluates chemicals. Although the standard method of manually scoring micronucleated (MNed) cells by microscope is a reliable and standard… read more here.
Keywords: tk6 cells; mode; h2ax foci; mode action ... See more keywords
3D-structured illumination microscopy reveals clustered DNA double-strand break formation in widespread γH2AX foci after high LET heavy-ion particle radiation
Sign Up to like & getrecommendations! Published in 2017 at "Oncotarget"
DOI: 10.18632/oncotarget.22679
Abstract: DNA double-strand breaks (DSBs) induced by ionising radiation are considered the major cause of genotoxic mutations and cell death. While DSBs are dispersed throughout chromatin after X-rays or γ-irradiation, multiple types of DNA damage including… read more here.
Keywords: h2ax foci; dna; microscopy; formation ... See more keywords