Loss of H3K27 trimethylation by immunohistochemistry is frequent in oligodendroglioma, IDH-mutant and 1p/19q-codeleted, but is neither a sensitive nor a specific marker
Sign Up to like & getrecommendations! Published in 2020 at "Acta Neuropathologica"
DOI: 10.1007/s00401-019-02123-8
Abstract: The integrated diagnosis for infiltrating gliomas requires demonstration of IDH1 or IDH2 mutation and 1p/19q codeletion in the tumor to diagnose oligodendroglioma or anaplastic oligodendroglioma, IDH-mutant and 1p/19q-codeleted. IDH1 R132H, ATRX and p53 immunohistochemistry may… read more here.
Keywords: immunohistochemistry; gliomas; idh mutant; h3k27me3 ... See more keywords
Quantitative Multiplexed ChIP Reveals Global Alterations that Shape Promoter Bivalency in Ground State Embryonic Stem Cells
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DOI: 10.1016/j.celrep.2019.08.046
Abstract: Summary To understand the epigenomic foundation of naive pluripotency, we implement a quantitative multiplexed chromatin immunoprecipitation sequencing (ChIP-seq) method comparing mouse embryonic stem cells (ESCs) grown in 2i versus 2i/serum and serum conditions. MINUTE-ChIP has… read more here.
Keywords: quantitative multiplexed; ground state; h3k27me3; embryonic stem ... See more keywords
Delayed Accumulation of H3K27me3 on Nascent DNA Is Essential for Recruitment of Transcription Factors at Early Stages of Stem Cell Differentiation.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.03.006
Abstract: Recruitment of transcription factors (TFs) to repressed genes in euchromatin is essential to activate new transcriptional programs during cell differentiation. However, recruitment of all TFs, including pioneer factors, is impeded by condensed H3K27me3-containing chromatin. Single-cell… read more here.
Keywords: cell differentiation; dna; h3k27me3; differentiation ... See more keywords
Replicational Dilution of H3K27me3 in Mammalian Cells and the Role of Poised Promoters.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.01.017
Abstract: Polycomb repressive complex 2 (PRC2) places H3K27me3 at developmental genes and is causally implicated in keeping bivalent genes silent. It is unclear if that silence requires minimum H3K27me3 levels and how the mark transmits faithfully… read more here.
Keywords: replicational dilution; poised promoters; h3k27me3; dilution h3k27me3 ... See more keywords
BMSC-EVs regulate Th17 cell differentiation in UC via H3K27me3.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular immunology"
DOI: 10.1016/j.molimm.2019.12.019
Abstract: In our previous studies, we found that extracellular vesicles in mesenchymal stem cells can alleviate ulcerative colitis. In view of the fact that extracellular vesicles have the same immunomodulatory effects as their maternal cells and… read more here.
Keywords: extracellular vesicles; h3k27me3; ulcerative colitis; differentiation ... See more keywords
C10ORF12 modulates PRC2 histone methyltransferase activity and H3K27me3 levels
Sign Up to like & getrecommendations! Published in 2019 at "Acta Pharmacologica Sinica"
DOI: 10.1038/s41401-019-0247-3
Abstract: The polycomb repressive complex 2 (PRC2) catalyzes the methylation of histone H3 on lysine 27 (H3K27) to generate trimethyl-H3K27 (H3K27me3) marks, thereby leading to a repressive chromatin state that inhibits gene expression. C10ORF12 was recently… read more here.
Keywords: histone methyltransferase; h3k27me3 levels; methyltransferase activity; h3k27me3 ... See more keywords
PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome
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DOI: 10.1038/s41467-020-20242-9
Abstract: The formation of zygote is the beginning of mammalian life, and dynamic epigenetic modifications are essential for mammalian normal development. H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional… read more here.
Keywords: prc2 ehmt1; establishment; h3k27me2 h3k27me3; h3k27me3 ... See more keywords
EZH2-mediated epigenetic silencing of TIMP2 promotes ovarian cancer migration and invasion
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-03362-z
Abstract: Enhancer of zeste homolog 2 (EZH2) is often increased in malignant tumors and is involved in metastasis. EZH2 silences gene expression by tri-methylating the lysine 27 residue of histone H3 (H3K27me3). However, the mechanism underlying… read more here.
Keywords: invasion; h3k27me3; ezh2; ovarian cancer ... See more keywords
Integrated analysis and transcript abundance modelling of H3K4me3 and H3K27me3 in developing secondary xylem
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DOI: 10.1038/s41598-017-03665-1
Abstract: Despite the considerable contribution of xylem development (xylogenesis) to plant biomass accumulation, its epigenetic regulation is poorly understood. Furthermore, the relative contributions of histone modifications to transcriptional regulation is not well studied in plants. We… read more here.
Keywords: secondary xylem; h3k4me3 h3k27me3; h3k27me3; xylem ... See more keywords
EZH1/2 inhibition augments the anti-tumor effects of sorafenib in hepatocellular carcinoma
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DOI: 10.1038/s41598-021-00889-0
Abstract: Both EZH2 and its homolog EZH1 function as histone H3 Lysine 27 (H3K27) methyltransferases and repress the transcription of target genes. Dysregulation of H3K27 trimethylation (H3K27me3) plays an important role in the development and progression… read more here.
Keywords: hepatocellular carcinoma; h3k27me3 levels; ezh1; sorafenib ... See more keywords
Role of Histone H3K27 Trimethylation Loss as a Marker for Malignant Peripheral Nerve Sheath Tumor in Fine-Needle Aspiration and Small Biopsy Specimens
Sign Up to like & getrecommendations! Published in 2017 at "American Journal of Clinical Pathology"
DOI: 10.1093/ajcp/aqx060
Abstract: Objectives Accurate diagnosis of malignant peripheral nerve sheath tumor (MPNST) is often challenging on fine-needle aspiration (FNA) or core needle biopsy. Recurrent mutations in EED and SUZ12, which encode subunits of polycomb repressive complex 2… read more here.
Keywords: biopsy; malignant peripheral; loss; h3k27me3 ... See more keywords