A turn in species conservation for hairpin banksias: demonstration of oversplitting leads to better management of diversity
Sign Up to like & getrecommendations! Published in 2022 at "American Journal of Botany"
DOI: 10.1002/ajb2.16074
Abstract: Abstract Premise Understanding evolutionary history and classifying discrete units of organisms remain overwhelming tasks, and lags in this workload concomitantly impede an accurate documentation of biodiversity and conservation management. Rapid advances and improved accessibility of… read more here.
Keywords: turn species; diversity; management; morphological diversity ... See more keywords
Hairpin-Bisulfite PCR.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_22
Abstract: Ligation of a hairpin oligonucleotide to genomic DNA prior to bisulfite conversion and PCR amplification physically links the two complementary DNA strands. This additional step in the conversion procedure overcomes the limitations of conventional bisulfite… read more here.
Keywords: hairpin bisulfite; bisulfite pcr; dna; hairpin ... See more keywords
Multiplex PCR with the blunt hairpin primers for next generation sequencing
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-017-0133-0
Abstract: The multiplex PCR is one of the important methods to enrich the target DNAs for next generation sequencing. The non-specific amplification and interaction between the primers are the pivotal challenges of multiplex PCR. Here, we… read more here.
Keywords: blunt hairpin; hairpin primers; hairpin; multiplex pcr ... See more keywords
A double signal amplification-based homogeneous electrochemical sensor built on catalytic hairpin assembly and bisferrocene markers.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114140
Abstract: A facile, sensitive and unmodified Hg2+ homogeneous electrochemical sensor based on bisferrocene signal markers and catalytic hairpin self-assembly (CHA) was built on a gold disk electrode. Three hairpin probes were designed, in which thiol was… read more here.
Keywords: hg2; probe; homogeneous electrochemical; hairpin ... See more keywords
Design and structural characterisation of monomeric water-soluble α-helix and β-hairpin peptides: State-of-the-art.
Sign Up to like & getrecommendations! Published in 2019 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2018.11.014
Abstract: Peptides are not only useful models for the structural understanding of protein folding and stability but also provide promising therapeutic avenues for the treatment of numerous diseases, and as biomaterials. The field has been very… read more here.
Keywords: water soluble; hairpin; structural characterisation; hairpin peptides ... See more keywords
An enzyme-free surface plasmon resonance imaging biosensing method for highly sensitive detection of microRNA based on catalytic hairpin assembly and spherical nucleic acid.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.02.055
Abstract: MicroRNAs (miRNAs), considered as therapeutic targets and biomarkers, play important roles in biological processes. Herein, an enzyme-free surface plasmon resonance imaging (SPRi) biosensing method has been developed for miRNA detection based on catalytic hairpin assembly… read more here.
Keywords: spherical nucleic; surface; hairpin; biosensing method ... See more keywords
Mismatch-fueled catalytic hairpin assembly mediated ultrasensitive biosensor for rapid detection of MicroRNA.
Sign Up to like & getrecommendations! Published in 2022 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2022.339663
Abstract: Herein, a mismatch-fueled catalytic hairpin assembly (MCHA) was rationally engineered, which possessed higher amplification efficiency and faster rate than catalytic hairpin assembly (CHA). Once input target microRNA-21(miRNA-21) triggers the MCHA, the hairpin DNA H1 will… read more here.
Keywords: hairpin; hairpin assembly; catalytic hairpin; mismatch fueled ... See more keywords
A novel β-hairpin peptide derived from the ARC repressor selectively interacts with the major groove of B-DNA.
Sign Up to like & getrecommendations! Published in 2021 at "Bioorganic chemistry"
DOI: 10.1016/j.bioorg.2021.104836
Abstract: Transcription factors (TFs) have a remarkable role in the homeostasis of the organisms and there is a growing interest in how they recognize and interact with specific DNA sequences. TFs recognize DNA using a variety… read more here.
Keywords: peptide; dna; hairpin; major groove ... See more keywords
Computational design of the helical hairpin structure of membrane-active antibacterial peptides based on RSV glycoprotein epitope scaffold
Sign Up to like & getrecommendations! Published in 2018 at "Computational biology and chemistry"
DOI: 10.1016/j.compbiolchem.2018.02.013
Abstract: Peptides with helical hairpin conformation have been found to possess potent membrane activity and can be exploited as the structural scaffold of antibacterial peptides (ABPs). Here, we attempted to computationally design membrane-active ABPs based on… read more here.
Keywords: membrane active; hairpin; rsv glycoprotein; antibacterial peptides ... See more keywords
MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins through the Action of the ERH Protein.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.01.026
Abstract: Microprocessor initiates the processing of microRNAs (miRNAs) from the hairpin regions of primary transcripts (pri-miRNAs). Pri-miRNAs often contain multiple miRNA hairpins, and this clustered arrangement can assist in the processing of otherwise defective hairpins. We… read more here.
Keywords: clustering assists; hairpin; helper hairpin; erh ... See more keywords
Ultrasensitive electrochemical detection of microRNA based on in-situ catalytic hairpin assembly actuated DNA tetrahedral interfacial probes.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122600
Abstract: Selective and sensitive detection of microRNA is crucial for early diagnosis and pathogenesis of disease. Here, we established a novel electrochemical biosensor for simple and accurate analysis of the tumor biomarker microRNA-141, which was based… read more here.
Keywords: situ catalytic; detection microrna; catalytic hairpin; detection ... See more keywords