Fast Kinetics Reveals Rate-Limiting Oxidation and the Role of the Aromatic Cage in the Mechanism of the Nicotine-Degrading Enzyme NicA2.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00855
Abstract: In Pseudomonas putida, the flavoprotein nicotine oxidoreductase (NicA2) catalyzes the oxidation of (S)-nicotine to N-methyl-myosmine, which is nonenzymatically hydrolyzed to pseudooxynicotine. Structural analysis reveals a monoamine oxidase (MAO)-like fold with a conserved FAD-binding domain and… read more here.
Keywords: mechanism; nica2; aromatic cage; half ... See more keywords
Structural mechanism for bacterial oxidation of oceanic trimethylamine into trimethylamine N‐oxide
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Microbiology"
DOI: 10.1111/mmi.13605
Abstract: Trimethylamine (TMA) and trimethylamine N‐oxide (TMAO) are widespread in the ocean and are important nitrogen source for bacteria. TMA monooxygenase (Tmm), a bacterial flavin‐containing monooxygenase (FMO), is found widespread in marine bacteria and is responsible… read more here.
Keywords: oxidation; mechanism; tmm; half reaction ... See more keywords