Enantioselectivity of haloalkane dehalogenase LinB on the degradation of 1,2-dichloropropane: A QM/MM study.
Sign Up to like & getrecommendations! Published in 2017 at "Bioorganic chemistry"
DOI: 10.1016/j.bioorg.2017.04.015
Abstract: The hydrolysis dechlorination mechanism of a chiral organochlorinepollutant, 1,2-dichloropropane (DCP), catalyzed by haloalkane dehalogenase LinB has been investigated by using a combined quantum mechanics/molecular mechanics method. LinB was confirmed to be enantioselective towards the catabolism… read more here.
Keywords: dichloropropane; haloalkane dehalogenase; dehalogenase linb; degradation dichloropropane ... See more keywords
A Haloalkane Dehalogenase from Saccharomonospora viridis Strain DSM 43017, a Compost Bacterium with Unusual Catalytic Residues, Unique (S)-Enantiopreference, and High Thermostability
Sign Up to like & getrecommendations! Published in 2020 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.02820-19
Abstract: The present study describes a novel haloalkane dehalogenase, DsvA, originating from a mildly thermophilic bacterium, Saccharomonospora viridis strain DSM 43017. We report its high thermostability, remarkable operational stability at high temperatures, and an (S)-enantiopreference, which… read more here.
Keywords: viridis strain; saccharomonospora viridis; halide; halide stabilizing ... See more keywords
Transient binding sites at the surface of haloalkane dehalogenase LinB as locations for fine-tuning enzymatic activity
Sign Up to like & getrecommendations! Published in 2023 at "PLOS ONE"
DOI: 10.1371/journal.pone.0280776
Abstract: The haloalkane dehalogenase LinB is a well-known enzyme that contains buried active site and is used for many modelling studies. Using classical molecular dynamics simulations of enzymes and substrates, we searched for transient binding sites… read more here.
Keywords: transient binding; haloalkane dehalogenase; sites surface; dehalogenase linb ... See more keywords
Description of Transport Tunnel in Haloalkane Dehalogenase Variant LinB D147C+L177C from Sphingobium japonicum
Sign Up to like & getrecommendations! Published in 2020 at "Catalysts"
DOI: 10.3390/catal11010005
Abstract: The activity of enzymes with active sites buried inside their protein core highly depends on the efficient transport of substrates and products between the active site and the bulk solvent. The engineering of access tunnels… read more here.
Keywords: d147c l177c; haloalkane dehalogenase; linb; linb d147c ... See more keywords
Crystallization and Crystallographic Analysis of a Bradyrhizobium Elkanii USDA94 Haloalkane Dehalogenase Variant with an Eliminated Halide-Binding Site
Sign Up to like & getrecommendations! Published in 2019 at "Crystals"
DOI: 10.3390/cryst9070375
Abstract: Haloalkane dehalogenases are a very important class of microbial enzymes for environmental detoxification of halogenated pollutants, for biocatalysis, biosensing and molecular tagging. The double mutant (Ile44Leu + Gln102His) of the haloalkane dehalogenase DbeA from Bradyrhizobium… read more here.
Keywords: halide binding; bradyrhizobium elkanii; elkanii usda94; haloalkane ... See more keywords