Predicting the Substrate Scope of the Flavin‐Dependent Halogenase BrvH
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DOI: 10.1002/cbic.202000444
Abstract: The recently described flavin‐dependent halogenase BrvH is able to catalyse both the bromination and chlorination of indole, but shows significantly higher bromination activity. BrvH was annotated as a tryptophan halogenase, but does not accept tryptophan… read more here.
Keywords: flavin dependent; halogenase; dependent halogenase; model ... See more keywords
Development of Halogenase Enzymes for Use in Synthesis.
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DOI: 10.1021/acs.chemrev.7b00032
Abstract: Nature has evolved halogenase enzymes to regioselectively halogenate a diverse range of biosynthetic precursors, with the halogens introduced often having a profound effect on the biological activity of the resulting natural products. Synthetic endeavors to… read more here.
Keywords: development halogenase; halogenase enzymes; enzymes use; halogenase ... See more keywords
Directed Evolution of an FeII-Dependent Halogenase for Asymmetric C(sp3)–H Chlorination
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DOI: 10.1021/acscatal.9b04691
Abstract: By using structure-guided directed evolution, the substrate scope of the FeII and α-ketoglutarate dependent halogenase Wi-WelO15 from Westiella intricata HT-29-1 was engineered to enable chemo-, regio-, and diastereoselective chlorination of unactivated C(sp3)–H bonds using NaCl… read more here.
Keywords: feii dependent; chlorination; halogenase; feii ... See more keywords
Halogenation of Peptides and Proteins Using Engineered Tryptophan Halogenase Enzymes
Sign Up to like & getrecommendations! Published in 2022 at "Biomolecules"
DOI: 10.3390/biom12121841
Abstract: Halogenation of bioactive peptides via incorporation of non-natural amino acid derivatives during chemical synthesis is a common strategy to enhance functionality. Bacterial tyrptophan halogenases efficiently catalyze regiospecific halogenation of the free amino acid tryptophan, both… read more here.
Keywords: halogenase enzymes; halogenation; halogenase; proteins using ... See more keywords