A high-throughput screening assay for the directed evolution-guided discovery of halohydrin dehalogenase mutants for epoxide ring-opening reaction.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2020.02.007
Abstract: Halohydrin dehalogenases (HHDHs) are valuable biocatalysts involved in the synthesis of β-substituted alcohols via their nucleophile-mediated ring-opening activity. To use directed evolution to unleash the latent potential of HHDHs for the synthesis of β-substituted alcohols,… read more here.
Keywords: ring opening; high throughput; directed evolution; halohydrin dehalogenase ... See more keywords
Origin of Chemoselectivity of Halohydrin Dehalogenase-Catalyzed Epoxide Ring-Opening Reactions
Sign Up to like & getrecommendations! Published in 2024 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.4c00640
Abstract: By performing molecular dynamics (MD), quantum mechanical/molecular mechanical (QM/MM) calculations, and QM cluster calculations, the origin of chemoselectivity of halohydrin dehalogenase (HHDH)-catalyzed ring-opening reactions of epoxide with the nucleophilic reagent NO2- has been explored. Four… read more here.
Keywords: halohydrin dehalogenase; opening reactions; ring opening; chemoselectivity ... See more keywords