HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye
Sign Up to like & getrecommendations! Published in 2021 at "ChemBioChem"
DOI: 10.1002/cbic.202100424
Abstract: HaloTag is a small self‐labeling protein that is frequently used for creating fluorescent reporters in living cells. The small‐molecule dyes used with HaloTag are almost exclusively based on rhodamine scaffolds, which are often expensive and… read more here.
Keywords: halotag engineering; halotag; engineering; engineering enhanced ... See more keywords
HaloTag™: Evaluation of a covalent one-step immobilization for biocatalysis.
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DOI: 10.1016/j.jbiotec.2016.12.004
Abstract: Easy, fast and gentle immobilization for the efficient reuse of important biocatalysts is highly demanded. We used the commercially available HaloTag™ technology (Promega), so far relatively unknown in the context of biocatalysis, to immobilize the… read more here.
Keywords: immobilization; evaluation covalent; biocatalysis; covalent one ... See more keywords